Comparative studies on amniotic fluid and plasma fibronectins
- Document Type:
- Reference
- Product Catalog Number:
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- Multiple
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Comparative studies on amniotic fluid and plasma fibronectins
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Intestinal epithelial wound healing assay in an epithelial-mesenchymal co-culture system.
- Rapid and efficient healing of epithelial damage is critical to the functional integrity of the small intestine. Epithelial repair is a complex process that has largely been studied in cultured epithelium but to a much lesser extent in mucosa. We describe a novel method for the study of wound healing using a co-culture system that combined an intestinal epithelial Caco-2/15 cell monolayer cultured on top of human intestinal myofibroblasts, which together formed a basement membrane-like structure that contained many of the major components found at the epithelial-mesenchymal interface in the human intestine. To investigate the mechanism of restitution, small lesions were generated in epithelial cell monolayers on plastic or in co-cultures without disturbing the underlying mesenchymal layer. Monitoring of wound healing showed that repair was more efficient in Caco-2/15-myofibroblast co-cultures than in Caco-2/15 monolayers and involved the deposition of basement membrane components. Functional experiments showed that the addition of type I collagen or human fibronectin to the culture medium significantly accelerated wound closure on epithelial cell co-cultures. This system may provide a new tool to investigate the mechanisms that regulate wound healing in the intestinal epithelium.
- Document Type:
- Reference
- Product Catalog Number:
- Multiple
- Product Catalog Name:
- Multiple
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HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2361790
- Document Type:
- Certificate of Analysis
- Lot Number:
- 2361790
- Product Catalog Number:
- FC010
- Product Catalog Name:
- Human Plasma Fibronectin Purified Protein
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HUMAN PLASMA FIBRONECTIN - 3893332
- Document Type:
- Certificate of Analysis
- Lot Number:
- 3893332
- Product Catalog Number:
- FC010
- Product Catalog Name:
- Human Plasma Fibronectin Purified Protein
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HUMAN PLASMA FIBRONECTIN - 3923918
- Document Type:
- Certificate of Analysis
- Lot Number:
- 3923918
- Product Catalog Number:
- FC010
- Product Catalog Name:
- Human Plasma Fibronectin Purified Protein
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HUMAN PLASMA FIBRONECTIN - 3383980
- Document Type:
- Certificate of Analysis
- Lot Number:
- 3383980
- Product Catalog Number:
- FC010
- Product Catalog Name:
- Human Plasma Fibronectin Purified Protein
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HUMAN PLASMA FIBRONECTIN -2645417
- Document Type:
- Certificate of Analysis
- Lot Number:
- 2645417
- Product Catalog Number:
- FC010
- Product Catalog Name:
- Human Plasma Fibronectin Purified Protein
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HUMAN PLASMA FIBRONECTIN - 4022807
- Document Type:
- Certificate of Analysis
- Lot Number:
- 4022807
- Product Catalog Number:
- FC010
- Product Catalog Name:
- Human Plasma Fibronectin Purified Protein
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HUMAN PLASMA FIBRONECTIN - 3065572
- Document Type:
- Certificate of Analysis
- Lot Number:
- 3065572
- Product Catalog Number:
- FC010
- Product Catalog Name:
- Human Plasma Fibronectin Purified Protein
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HUMAN PLASMA FIBRONECTIN - 3272821
- Document Type:
- Certificate of Analysis
- Lot Number:
- 3272821
- Product Catalog Number:
- FC010
- Product Catalog Name:
- Human Plasma Fibronectin Purified Protein