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hgammag-301k

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HGAMMAG-301K xPonent Protocol Template Version 3.1
Document Type:

Technical Information
HGAMMAG-301K xPonent Standard Template Version Magpix
Document Type:

Technical Information
HGAMMAG-301K xPonent Protocol Template Version Magpix
Document Type:

Technical Information
Protocol- HGAMMAG-301K
Document Type:

Protocols
The Power of Biomarker Analysis
Document Type:

Brochure

Product Catalog Number:

Multiple
Product Catalog Name:

Multiple
Detection of immunoglobulin isotypes from dried blood spots.
The study was designed to determine the sensitivity and reproducibility of recovering immunoglobulin (Ig) isotypes (IgG subclasses, IgA, IgE and IgM classes) from dried blood spots (DBS), a methodologic subcomponent of the Upstate KIDS Study. A multiplexed Luminex assay was used for IgG1/2/3/4, IgA and IgM analysis; an ELISA was used for IgE. Plasma samples from de-identified patients were used to compare the Luminex assay with nephelometry, which is routinely used to quantify IgA, IgG and IgM in clinical samples. The IgE ELISA was compared to an immunofluorescence assay. Prior to evaluation of punches from newborn dried blood spots (NDBSs), recoveries of Ig from punches of cord blood DBSs (CBDBSs) vs. plasma from the same cord bloods were compared. Although the recoveries of Ig from plasma and DBSs were not comparable, which could be due to cell lysates in the DBS samples, the analyses were reproducible. Additionally, the levels of IgA, IgG2, IgG4, and IgM recovered from CBDBSs positively correlated with those in plasma. The DBS data is a relative value since it is not equivalent to the plasma concentration. The majority of Ig concentrations recovered from 108 newborns of the Upstate KIDs Study were within the range of newborn plasma Ig levels with the exception of IgG3. The IgG4 values displayed the greatest variance with a wide range (0.01-319 mg/dl), whereas, IgG1 values had the narrowest range (85.2-960.4 mg/dl).
Document Type:

Reference

Product Catalog Number:

HGAMMAG-301K
Product Catalog Name:

MILLIPLEX® MAP Human Isotyping Magnetic Bead Panel - Isotyping Multiplex Assay
Quality Control Ranges: Milliplex Human Immunoglobulin Isotyping Kit
Document Type:

Technical Information
96 well Template 2 Up
Document Type:

Technical Information
96 well Template Single
Document Type:

Technical Information