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  • Anti-Chymase, clone B7 - 4194306

    Document Type:
    Certificate of Analysis
    Lot Number:
    4194306
    Product Catalog Number:
    MAB1254
    Product Catalog Name:
    Anti-Chymase Antibody, clone B7

  • Expression of angiotensin II and interleukin 6 in human coronary atherosclerotic plaques: potential implications for inflammation and plaque instability. 10736279

    BACKGROUND: Patients with an activated renin-angiotensin system (RAS) or genetic alterations of the RAS are at increased risk of myocardial infarction (MI). Administration of ACE inhibitors reduces the risk of MI, and acute coronary syndromes are associated with increased interleukin 6 (IL-6) serum levels. Accordingly, the present study evaluated the expression of angiotensin II (Ang II) in human coronary atherosclerotic plaques and its influence on IL-6 expression in patients with coronary artery disease. METHODS AND RESULTS: Immunohistochemical colocalization of Ang II, ACE, Ang II type 1 (AT(1)) receptor, and IL-6 was examined in coronary arteries from patients with ischemic or dilated cardiomyopathy undergoing heart transplantation (n=12), in atherectomy samples from patients with unstable angina (culprit lesion; n=8), and in ruptured coronary arteries from patients who died of MI (n=13). Synthesis and release of IL-6 was investigated in smooth muscle cells and macrophages after Ang II stimulation. Colocalization of ACE, Ang II, AT(1) receptor, and IL-6 with CD68-positive macrophages was observed at the shoulder region of coronary atherosclerotic plaques and in atherectomy tissue of patients with unstable angina. Ang II was identified in close proximity to the presumed rupture site of human coronary arteries in acute MI. Ang II induced synthesis and release of IL-6 shortly after stimulation in vitro in macrophages and rat smooth muscle cells. CONCLUSIONS: Ang II, AT(1) receptor, and ACE are expressed at strategic sites of human atherosclerotic coronary arteries, suggesting that Ang II is produced primarily by ACE within coronary plaques. The observation that Ang II induces IL-6 and their colocalization with the AT(1) receptor and ACE is consistent with the notion that the RAS may contribute to inflammatory processes within the vascular wall and to the development of acute coronary syndromes.
    Document Type:
    Reference
    Product Catalog Number:
    MAB1254
    Product Catalog Name:
    Anti-Chymase Antibody, clone B7

  • Anti-Chymase, clone B7 - 4293969

    Document Type:
    Certificate of Analysis
    Lot Number:
    4293969
    Product Catalog Number:
    MAB1254
    Product Catalog Name:
    Anti-Chymase Antibody, clone B7

  • Anti-Chymase, clone B7 - 3862705

    Document Type:
    Certificate of Analysis
    Lot Number:
    3862705
    Product Catalog Number:
    MAB1254
    Product Catalog Name:
    Anti-Chymase Antibody, clone B7

  • Anti-Chymase, clone B7 - NG1859617

    Document Type:
    Certificate of Analysis
    Lot Number:
    NG1859617
    Product Catalog Number:
    MAB1254
    Product Catalog Name:
    Anti-Chymase Antibody, clone B7

  • Anti-Chymase, clone B7 - 4297432

    Document Type:
    Certificate of Analysis
    Lot Number:
    4297432
    Product Catalog Number:
    MAB1254
    Product Catalog Name:
    Anti-Chymase Antibody, clone B7

  • Genome-wide gene expression profiling of human mast cells stimulated by IgE or FcepsilonRI-aggregation reveals a complex network of genes involved in inflammatory respons ... 16911805

    BACKGROUND: Mast cells are well established effectors of IgE-triggered allergic reactions and immune responses to parasitic infections. Recent studies indicate that mast cells may play roles in adaptive and innate immunity, suggesting an innovative view of the regulation of immune responses. Here, we profiled the transcriptome of human mast cells sensitized with IgE alone, or stimulated by FcepsilonRI aggregation. RESULTS: Our data show that among 8,793 genes examined, 559 genes are differentially regulated in stimulated mast cells when compared with resting/unstimulated mast cells. The major functional categories of upregulated genes include cytokines, chemokines, and other genes involved in innate and adaptive immune-responses. We observed the increased expression of over 63 gene-transcripts following IgE-sensitization alone. Our data was validated using Real-Time-PCR; ELISA and western blot. We confirmed that IgE alone does not trigger mast cell-immediate responses, such as calcium signals, degranulation or protein-phosphorylation. CONCLUSION: This report represents a substantial advance in our understanding of the genome wide effects triggered by passive sensitization or active stimulation of human mast cells, supporting mast cells' potential involvement in a wide range of inflammatory responses.
    Document Type:
    Reference
    Product Catalog Number:
    MAB1254
    Product Catalog Name:
    Anti-Chymase Antibody, clone B7

  • Anti-Chymase, clone B7 - 3427288

    Document Type:
    Certificate of Analysis
    Lot Number:
    3427288
    Product Catalog Number:
    MAB1254
    Product Catalog Name:
    Anti-Chymase Antibody, clone B7

  • Anti-Chymase, clone B7 -2594321

    Document Type:
    Certificate of Analysis
    Lot Number:
    2594321
    Product Catalog Number:
    MAB1254
    Product Catalog Name:
    Anti-Chymase Antibody, clone B7