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  • A monoclonal antibody against neurofilament protein specifically labels a subpopulation of rat sensory neurones. 6434599

    A monoclonal antibody (RT97) against neurofilament protein specifically and exclusively labelled a subpopulation of rat dorsal root ganglion (DRG) neurones. For seven ganglia (L4 and T13) studied quantitatively the frequency distribution histograms of the size of labelled cells could be fitted by a single normal distribution whose parameters were extremely close to those of the normally distributed large light cell population in that ganglion. On this basis and on the basis of a statistical analysis of the results it was suggested that this antibody can be used as a much needed specific label for the large light population of neurones in rat DRGs. The small dark neurone population was not labelled by this antibody. In one ganglion the subjective analysis of whether each neurone was labelled or not was directly compared with microdensitometric measurements of reaction product intensity. This analysis supported the above conclusion, and furthermore no subdivisions of the labelled population were apparent on the basis of neuronal size plotted against intensity of the reaction product. Other neuronal cell bodies strongly labelled by this antibody were found in association with small unlabelled neurones not only in DRGs, but also in the trigeminal ganglion, the vagal ganglia, and the mesencephalic V nucleus, all of which are made up of primary afferent neurones and all of which are completely or partially derived from the neural crest. Sympathetic and central nervous system neuronal cell bodies were unlabelled or occasionally very lightly labelled although immunoreactive fibres abound in the central nervous system.
    Document Type:
    Reference
    Product Catalog Number:
    MAB5262
    Product Catalog Name:
    Anti-Neurofilament 200 kDa Antibody, clone RT97

  • A monoclonal antibody TrkB receptor agonist as a potential therapeutic for Huntington's disease. 24503862

    Huntington's disease (HD) is a devastating, genetic neurodegenerative disease caused by a tri-nucleotide expansion in exon 1 of the huntingtin gene. HD is clinically characterized by chorea, emotional and psychiatric disturbances and cognitive deficits with later symptoms including rigidity and dementia. Pathologically, the cortico-striatal pathway is severely dysfunctional as reflected by striatal and cortical atrophy in late-stage disease. Brain-derived neurotrophic factor (BDNF) is a neuroprotective, secreted protein that binds with high affinity to the extracellular domain of the tropomyosin-receptor kinase B (TrkB) receptor promoting neuronal cell survival by activating the receptor and down-stream signaling proteins. Reduced cortical BDNF production and transport to the striatum have been implicated in HD pathogenesis; the ability to enhance TrkB signaling using a BDNF mimetic might be beneficial in disease progression, so we explored this as a therapeutic strategy for HD. Using recombinant and native assay formats, we report here the evaluation of TrkB antibodies and a panel of reported small molecule TrkB agonists, and identify the best candidate, from those tested, for in vivo proof of concept studies in transgenic HD models.
    Document Type:
    Reference
    Product Catalog Number:
    07-225

  • LN-6: a monoclonal antibody to vimentin expressed in non-hematopoietic mesenchymal cells and derived tumors and reactive in B5-fixed, paraffin-embedded tissues. 2671152

    We generated a monoclonal antibody (MAb), designated LN-6, directed against human vimentin, which retains its immunoreactivity in B5-fixed, paraffin-embedded tissues. Like other anti-vimentin MAb, LN-6 was found to be reactive with a wide spectrum of human sarcomas and normal cells of mesenchymal derivation. However, unlike other similar reagents, LN-6 was unreactive with normal and malignant human lymphoid cells and therefore displays a more restricted immunoreactivity. Because of its ability to stain routinely processed pathological tissues and its marked reactivity with human sarcomas, LN-6 is a unique reagent for the immunohistochemical diagnosis of human cancer.
    Document Type:
    Reference
    Product Catalog Number:
    MAB1681
    Product Catalog Name:
    Anti-Vimentin Antibody, clone LN-6

  • A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. II. Effects on cell movement, organization of microtubules, and intermediate f ... 6685128

    A rat monoclonal antibody against yeast alpha-tubulin (clone YL 1/2; Kilmartin, J. V., B. Wright, and C. Milstein, 1982, J. Cell Biol., 93:576-582) that reacts specifically with the tyrosylated form of alpha-tubulin and readily binds to tubulin in microtubules when injected into cultured cells (see Wehland, J., M. C. Willingham, and I. V. Sandoval, 1983, J. Cell Biol., 97:1467-1475) was used to study microtubule organization and function in living cells. Depending on the concentration of YL 1/2 that was injected the following striking effects were observed: (a) When injected at a low concentration (2 mg IgG/ml in the injection solution), where microtubules were decorated without changing their distribution, intracellular movement of cell organelles (saltatory movement) and cell translocation were not affected. Intermediate concentrations (6 mg IgG/ml) that induced bundling but no perinuclear aggregation of microtubules abolished saltatory movement and cell translocation, and high concentrations (greater than 12 mg IgG/ml) that induced perinuclear aggregation of microtubules showed the same effect. (b) YL 1/2, when injected at intermediate and high concentrations, arrested cells in mitosis. Such cells showed no normal spindle structures. (c) Injection of an intermediate concentration of YL 1/2 that stopped saltatory movement caused little or no aggregation of intermediate filaments and no dispersion of the Golgi complex. After injection of high concentrations, resulting in perinuclear aggregation of microtubules, intermediate filaments formed perinuclear bundles and the Golgi complex became dispersed analogous to results obtained after treatment of cells with colcemid. (d) When rhodamine-conjugated YL 1/2 was injected at concentrations that stopped saltatory movement and arrested cells in mitosis, microtubule structures could be visualized and followed for several hours in living cells by video image intensification microscopy. They showed little or no change in distribution and organization during observation, even though these microtubule structures appeared not to be stabilized by injected YL 1/2 since they were readily depolymerized by colcemid or cold treatment and repolymerized upon drug removal or rewarming to 37 degrees C, respectively. These results are discussed in terms of the participation of microtubules in cellular activities such as cell movement and cytoplasmic organization and in terms of the specificity of YL 1/2 for the tyrosylated form of alpha-tubulin.
    Document Type:
    Reference
    Product Catalog Number:
    MAB1864-I
    Product Catalog Name:
    Anti-alpha-Tubulin Antibody, tyrosinated, clone YL1/2