Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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We provide gentle, membrane-based ProteinConcentration options to concentrate proteins without compromising yield, even when labile or low in abundance.
For filtering large volumes of biological solutions, we manufacture specialized equipment including centrifugal filtering devices, discs, cells and solvents.
For high throughput sample preparation, we provide ultrafiltration based filter plates designed for automation-compatible sample purification, protein removal, concentrating and de-salting.
From protein extraction and protein purification, to proteinconcentration and desalting, we facilitate every step of your sample preparation workflow with ultrafiltration devices, magnetic beads, extraction kits and more
This page describes common challenges encountered when lysing cells and extracting proteins prior to Western blotting. Total proteinconcentration must be determined for these cell lysates. Variables affecting each of these steps are outlined below, as each could affect the sensitivity and reproducibility of the Western blot.
Designed for clinical samples, Centrifree ultrafiltration devices separate free from protein-bound micro-solute in small volumes of serum, plasma, and other biological samples.
Technical information on the properties of ultrafiltration membranes and how they have been optimized for proteinconcentration and the fine separation of other macromolecular solutes.
The possible causes and potential remedies for challenges encountered in the immunoprecipitation-Western blot technique, which consists of cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.
Amicon Ultra 0.5 mL filters provide fast ultrafiltration with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices.