Fluoro-Gold: a new fluorescent retrograde axonal tracer with numerous unique properties
- Document Type:
- Reference
- Product Catalog Number:
- AB153
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Fluoro-Gold: a new fluorescent retrograde axonal tracer with numerous unique properties
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Commissurally projecting inhibitory interneurons of the rat hippocampal dentate gyrus: a colocalization study of neuronal markers and the retrograde tracer Fluoro-gold.
- Improved methods for detecting neuronal markers and the retrograde tracer Fluoro-Gold (FG) were used to identify commissurally projecting neurons of the rat hippocampus. In addition to the dentate hilar mossy cells and CA3 pyramidal cells shown previously to transport retrograde tracers after injection into the dorsal hippocampus, FG-positive interneurons of the dentate granule cell layer and hilus were detected in numbers greater than previously reported. FG labeling of interneurons was variable among animals, but was as high as 96% of hilar somatostatin-positive interneurons, 84% of parvalbumin-positive cells of the granule cell layer and hilus combined, and 33% of hilar calretinin-positive cells. By comparison, interneurons of the dentate molecular layer and all hippocampal subregions were conspicuously FG-negative. Whereas hilar mossy cells and CA3 pyramidal cells were FG-labeled throughout the longitudinal axis, FG-positive interneurons exhibited a relatively homotopic distribution. Control injections of FG into the neocortex, septum, and ventral hippocampus demonstrated that the homotopic labeling of dentate interneurons was injection site-specific, and that the CA1-CA3 interneurons unlabeled by contralateral hippocampal FG injection were nonetheless able to transport FG from the septum. These data suggest a hippocampal organizing principle according to which virtually all commissurally projecting hippocampal neurons share the property of being monosynaptic targets of dentate granule cells. Because granule cells innervate their exclusively ipsilateral target cells in a highly lamellar pattern, these results suggest that focal granule cell excitation may result in commissural inhibition of the corresponding twin granule cell lamella, thereby lateralizing and amplifying the influence of the initiating discharge.
- Document Type:
- Reference
- Product Catalog Number:
- Multiple
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LuminiCell Tracker 540- Vascular Labeling Kit - VP1905290
- Document Type:
- Certificate of Analysis
- Lot Number:
- VP1905290
- Product Catalog Number:
- SCT012
- Product Catalog Name:
- LuminiCell Tracker™ 540- Vascular Labeling Kit
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LuminiCell Tracker 540- Cell Labeling Kit - VP1908270
- Document Type:
- Certificate of Analysis
- Lot Number:
- VP1908270
- Product Catalog Number:
- SCT010
- Product Catalog Name:
- LuminiCell Tracker™ 540- Cell Labeling Kit
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LuminiCell Tracker 540- Cell Labeling Kit - VP1905291
- Document Type:
- Certificate of Analysis
- Lot Number:
- VP1905291
- Product Catalog Number:
- SCT010
- Product Catalog Name:
- LuminiCell Tracker™ 540- Cell Labeling Kit
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LuminiCell Tracker 670- Cell Labeling Kit - VP1905134
- Document Type:
- Certificate of Analysis
- Lot Number:
- VP1905134
- Product Catalog Number:
- SCT011
- Product Catalog Name:
- LuminiCell Tracker™ 670- Cell Labeling Kit
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LuminiCell Tracker¿ 540- Vascular Labeling Kit
- Document Type:
- Data Sheet
- Product Catalog Number:
- SCT012
- Product Catalog Name:
- LuminiCell Tracker™ 540- Vascular Labeling Kit
-
LuminiCell Tracker¿ 670- Vascular Labeling Kit
- Document Type:
- Data Sheet
-
LuminiCell Tracker¿ 540- Cell Labeling Kit
- Document Type:
- Data Sheet
-
LuminiCell Tracker 670- Vascular Labeling Kit
- Document Type:
- Certificate of Analysis
- Lot Number:
- VP1707121
- Product Catalog Number:
- SCT013
- Product Catalog Name:
- LuminiCell Tracker™ 670- Vascular Labeling Kit