537088
Pronase® Protease, Streptomyces griseus, Nuclease-Free
Widely used broad-spectrum protease mixture
別名:
Nuclease-Free Protease, Streptomyces griseus, Pronase® Protease, Pronase® Protease, Streptomyces griseus
製品名
Pronase® Protease, Streptomyces griseus, Nuclease-Free, Widely used broad-spectrum protease mixture
description
RTECS - UK9595000
form
lyophilized
specific activity
≥70,000 proteolytic units/g dry wt
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
water: 10 mg/mL
foreign activity
Nuclease, none detected
shipped in
ambient
storage temp.
2-8°C
Application
Pronase® Protease, Streptomyces griseus, nuclease-free has been used:
- in single-cell preparations for cardiac side population (CSP) analysis
- in aflatoxin plasma biomarker assay
- to permeabilize Caenorhabditis elegans embryo for in vivo micrococcal nuclease digestion
Biochem/physiol Actions
Proteases catalyze the hydrolysis of proteins to form peptides and free amino acids. They also regulate the breakdown of fats and carbohydrates through many enzymatic cascades and activating proenzyme zymogens.
Disclaimer
Toxicity: Harmful (C)
General description
PRONASE® Protease, Streptomyces griseus, nuclease-free is a non-specific protease that liquefies mucins and digests proteins to free amino acids. This mixture can be used to isolate living chondrocytes. It is most active at pH 7.0 but stable over wide ranges of pH and temperature. It has an optimal pH of 7.5. Pronase is less cytotoxic and produces more efficient dissociation than collagenase.
Other Notes
Note: 1 KU = 1000 units.
One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
Preparation Note
Unstable in solution; reconstitute just prior to use.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
保管分類
11 - Combustible Solids
wgk
WGK 2
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Ehsan Pourkarimi et al.
eLife, 5 (2016-12-24)
The primary task of developing embryos is genome replication, yet how DNA replication is integrated with the profound cellular changes that occur through development is largely unknown. Using an approach to map DNA replication at high resolution in C. elegans
Mustafa Mahfuz et al.
Journal of exposure science & environmental epidemiology, 29(5), 655-662 (2018-09-07)
Dietary exposure to aflatoxin is implicated in growth faltering of children. Despite the high burden of childhood stunting in urban Bangladesh, there are no data on long-term exposure to aflatoxin. This study aimed to explore aflatoxin exposure levels in a
Industrial Biotechnology and Commodity Products
Comprehensive Biotechnology, 3, 587-603 null
Annette Meeson et al.
Stem cell reviews and reports, 9(5), 731-739 (2013-04-27)
Cardiac side population (CSP) cells, defined by their ability to efflux the vital dye Hoechst 33342, have been identified as putative cardiac stem cells based on their potential to give rise to both cardiomyocytes and endothelial cells. The CSP phenotype
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 537088-50KUCN | 04055977195996 |
| 537088-25KUCN | 04055977195972 |
| 537088-10KUCN | 04055977195958 |
| 537088-5KUCN | 04055977195989 |
| 537088-100KUCN | 04055977195965 |
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