05-1144
Anti-Talin Antibody, CT, clone TD77
clone TD77, from mouse
別名:
talin 1
製品名
Anti-Talin Antibody, CT, clone TD77, clone TD77, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
TD77, monoclonal
species reactivity
chicken, human, mouse
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
chicken ... Tln1(395194)
human ... TLN1(7094)
mouse ... Tln1(21894)
General description
Talin consisting of a 47-kDa N-terminal head domain (residues 1-433) and a 190-kDa
C-terminal rod domain (residues 434-2541), links integrins to the actin cytoskeleton.
C-terminal rod domain (residues 434-2541), links integrins to the actin cytoskeleton.
270 kDa
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB3264
Physical form
Format: Purified
Purified from Protein A Sepharose™ chromatography
Purified immunoglobulin from Protein A Sepharose™ chromatography. Liquid in 0.02M PBS pH 7.6, 0.25 M NaCl containing 0.1% sodium azide.
Preparation Note
Maintain between 2 and 8°C for 1 year after date of receipt.
Legal Information
Sepharose is a trademark of Cytiva
Analysis Note
Evaluated by Western Blot in Jurkat cell lysates.
Western Blot Analysis: A 1:500 dilution of this lot detected Talin in 10 ug of Jurkat lysates.
Western Blot Analysis: A 1:500 dilution of this lot detected Talin in 10 ug of Jurkat lysates.
Application
Detect Talin using this Anti-Talin Antibody, C-terminus, clone TD77 validated for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Talin in human GI track tissue.
Biochem/physiol Actions
Expected cross-reactivity in mouse and chicken.
Recognizes Human Talin, and cross reacts with Mouse and chicken Talin. The epitope recognized by this antibody is located within the C-terminal region of the protein (residues 2269-2541), which also contains an F-actin binding site homologous to that in the yeast actin-binding protein SIa2p.
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保管分類
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
05-1144:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Michèle Moes et al.
The Journal of biological chemistry, 282(23), 17280-17288 (2007-04-14)
Talin1 is a large cytoskeletal protein that links integrins to actin filaments through two distinct integrin binding sites, one present in the talin head domain (IBS1) necessary for integrin activation and a second (IBS2) that we have previously mapped to
Andreas Wortmann et al.
The Journal of biological chemistry, 286(49), 42303-42315 (2011-10-14)
Reciprocal interactions between Src family kinases (SFKs) and focal adhesion kinase (FAK) are critical during changes in cell attachment. Recently it has been recognized that another SFK substrate, CUB-domain-containing protein 1 (CDCP1), is differentially phosphorylated during these events. However, the
Yekaterina B Khotskaya et al.
Molecular carcinogenesis, 53(12), 1011-1026 (2013-09-04)
Metastatic dissemination is a multi-step process that depends on cancer cells' ability to respond to microenvironmental cues by adapting adhesion abilities and undergoing cytoskeletal rearrangement. Breast Cancer Metastasis Suppressor 1 (BRMS1) affects several steps of the metastatic cascade: it decreases
Mayra Álvarez-Santos et al.
Journal of cellular biochemistry, 117(10), 2385-2396 (2016-03-13)
The extracellular domains of some membrane proteins can be shed from the cell. A similar phenomenon occurs with β1 integrins (α1β1 and α2β1) in guinea pig. The putative role of β1 integrin subunit alterations due to shedding in airway smooth
Michael C Schmid et al.
Nature communications, 13(1), 1768-1768 (2022-04-03)
Myeloid cells play key roles in cancer immune suppression and tumor progression. In response to tumor derived factors, circulating monocytes and granulocytes extravasate into the tumor parenchyma where they stimulate angiogenesis, immune suppression and tumor progression. Chemokines, cytokines and interleukins
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