524625
Phosphatase Inhibitor Cocktail II
liquid, for the inhibition of acid phosphatases, alkaline phosphatases and protein tyrosine phosphatases (PTP's). Suitable for use with cell lysates and tissue extracts.
別名:
Phosphatase Inhibitor, Phosphatase Inhibitor Solution (Set II)
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この商品について
UNSPSC Code:
12352200
NACRES:
NA.77
Form:
liquid
Solubility:
water: soluble
Storage temp.:
−20°C
製品名
Phosphatase Inhibitor Cocktail Set II, A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts.
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
water: soluble
shipped in
wet ice
storage temp.
−20°C
Quality Level
General description
A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts, including samples containing detergents.
Application
Phosphatase Inhibitor Cocktail Set II has been used:
- in triple detergent buffer to lyse cultured cells to extract total protein for western blots
- as a component of lysis buffer to lyse tissue samples or cultured rat ventricular cardiomyocytes (VCMs) for western blot analysis of cardiac mitochondrial proteins
- as a component of lysis buffer to lyse cultured rat VCMs infected with Adv-COX7RP and Adv-shRNA COX7RP for immunoprecipitation of ryanodine receptors type 2 (RyR2)
- as a component in ice-cold RIPA buffer to homogenize/lyse retina tissues for protein extraction and western blotting
Biochem/physiol Actions
Protein phosphorylation and dephosphorylation play essential roles in signal transduction processes that regulate various cellular functions including cell division, proliferation, and apoptosis. Protein kinases transfer phosphate groups to specific serine, threonine, or tyrosine residues, leading to a conformational change in the protein and affecting its interaction with ligands. Protein phosphatases reverse this process by removing the phosphate group and restoring the protein to its original dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain the phosphorylation state of the proteins intact.
Features and Benefits
- Flexible: compatible with cell lysates and tissue extracts, including samples containing detergents.
- Economical: ready-to-use format minimizes wastage.
- Effective: one ml is adequate for approximately 100 ml extraction buffer.
- Complete: contains five phosphatase inhibitors targeting acid and alkaline phosphatases and protein tyrosine phosphatases (PTPs).
Other Notes
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Provided as a set of five 1 ml vials containing the following components:
- Imidazole (200 mM)
- Sodium Fluoride (100 mM)
- Sodium Molybdate (115 mM)
- Sodium Orthovanadate (100 mM)
- Sodium Tartrate Dihydrate (400 mM)
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
保管分類
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Class I Designated Chemical Substances
prtr
Substances Subject to be Indicated Names
ishl_indicated
Substances Subject to be Notified Names
ishl_notified
524625-1.0ML: + 524625-1EA: + 524625-10ML: + 524625-100ML: + 524625-1SET:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Rapamycin and fasting sustain autophagy response activated by ischemia reperfusion injury and promote retinal ganglion cell survival
Russo R, et al.
Cell Death & Disease, 9, 1-18 (2018)
The Endolysosomal Transporter DMT1 is Required for Morphine Regulation of Neuronal Ferritin Heavy Chain
Irollo E, et al.
Journal of Neuroimmune Pharmacology, 18(3), 495-508 (2023)
Sexual dimorphism in bidirectional SR-mitochondria crosstalk in ventricular cardiomyocytes
Clements RT, et al.
Basic Research in Cardiology, 118, 1-22 (2023)
Aarthi Gunasekaran et al.
Pediatric research, 87(7), 1177-1184 (2019-09-10)
Disruption of tight junctions (TJs) predisposes to bacterial translocation, intestinal inflammation, and necrotizing enterocolitis (NEC). Previously, studies showed that hyaluronan (HA), a glycosaminoglycan in human milk, maintains intestinal permeability, enhances intestinal immunity, and reduces intestinal infections. In this study, we
Dane Malone et al.
The Journal of biological chemistry, 294(40), 14674-14685 (2019-08-10)
Human Schlafen 11 (SLFN11) is an interferon-stimulated gene (ISG) that we previously have demonstrated to ablate translation of HIV proteins based on the virus's distinct codon preference. Additionally, lack of SLFN11 expression has been linked to the resistance of cancer
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 524625-1SETCN | 07790788051068 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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