Protease Inhibitor Cocktail I

Protease Inhibitor Cocktail Set I – Calbiochem has been used in lysis buffer to lyse cells and perform western blotting.

Protease Inhibitor Cocktail Set I have been used as a supplement in:

• cold radioimmunoprecipitation assay (RIPA) buffer to prepare cell lysates for western blotting
• RIPA lysis buffer to extract total protein from rat liver tissues and AML12 cells
• sucrose solution to homogenize mice cortices for myelin isolation

Inhibits a broad range of proteases and esterases.

Protease inhibitor cocktails and specific protease inhibitors are essential tools utilized by scientists in various proteomic studies. These studies encompass protein expression, characterization, biomarker discovery, mapping post-translational modifications, protein expression profiling, and quantitative protein measurement. The application of a protease inhibitor cocktail or protease inhibitors guarantees the preservation of protein integrity, facilitating downstream analysis and characterization.

Toxicity: Harmful (C)

Protease Inhibitor Cocktail Set I A is specially formulated containing five protease inhibitors that will inhibit a broad range of proteases and esterases.

The presence of EDTA may interfere with the purification of His•Tag fusion proteins if a cell extract is applied directly to IMAC resins.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Reconstitute each vial with 1 ml dH2O to yield a 100X stock solution.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany