539134
Protease Inhibitor Cocktail III
EDTA-Free, liquid, for the inhibition of aspartic, cysteine, and serine proteases and aminopeptidases in mammalian cells and tissues.
別名:
EDTA free Protease inhibitor, EDTA-Free Protease Inhibitor Mix, Protease inhibitor cocktail III
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この商品について
UNSPSC Code:
12352200
NACRES:
NA.77
Form:
liquid
Solubility:
water: soluble
Storage temp.:
−20°C
製品名
Protease Inhibitor Cocktail Set III, EDTA-Free, Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
water: soluble
shipped in
wet ice
storage temp.
−20°C
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General description
Note: available as 1 x 1 ml or as a set of 5 x 1 ml.
This protease inhibitor cocktail set is a specially formulated cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases. This cocktail is recommended for use with mammalian cells and tissue extracts, but is also suitable for bacterial extracts. It is provided as a single vial or as a set of 5 vials. Each vial contains 1 ml protease inhibitor cocktail solubilized in DMSO with a small amount of water.
Application
Protease Inhibitor Cocktail Set III, EDTA-Free has been used:
- as a component in CelLytic MT cell lysis reagent or lysis buffer to homogenize kidney organoids for immunoblot analysis
- as a component in homogenization buffer to homogenize minced mouse quadriceps femoris muscle tissue
- in lysis buffer to extract proteins for immunoblotting
Biochem/physiol Actions
Inhibits a broad spectrum of serine proteases, cysteine proteases, and aspartic proteases, as well as aminopeptidases.
Protease inhibitor cocktails and specialized protease inhibitors play a crucial role in various proteomic studies conducted by scientists. These studies encompass protein expression and characterization, biomarker discovery, identification of post-translational modifications, protein expression profiling, and quantitative protein measurement. The inclusion of a protease inhibitor cocktail or specific protease inhibitor guarantees the preservation of protein integrity, allowing for accurate downstream analysis and comprehensive sample characterization.
Features and Benefits
- EDTA-Free: recommended for IMAC applications.
- Flexible: compatible with bacterial extracts, mammalian cell extracts, and tissue extracts.
- Fast: no waiting time for tablets to dissolve.
- Economical: ready-to-use format reduces wastage.
- Effective: one ml is sufficient for approximately 20g of tissue.
- Complete: contains six protease inhibitors to inhibit aspartic, cysteine, serine proteases, and aminopeptidases.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
保管分類
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F - (refers to pure substance)
flash_point_c
87 °C - (refers to pure substance)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Group 4: Flammable liquids + Type 3 petroleums + Hazardous rank III + Water soluble liquid
fsl
Substances Subject to be Indicated Names
ishl_indicated
Substances Subject to be Notified Names
ishl_notified
539134-100ML: + 539134-1UNML: + US1539134-1SET: + 539134-10ML: + US1539134-1ML: + 539134-25SUL: + 539134-1ML: + 539134-1SET: + US2539134-1ML: + 539134-1SML: + 539134-1EA: + 539134-50SML: + 539134-50ML:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Wei-Ling Wang et al.
American journal of physiology. Renal physiology, 318(4), F956-F970 (2020-02-25)
Aquaporin-2 (AQP2) is a vasopressin-regulated water channel protein responsible for osmotic water reabsorption by kidney collecting ducts. In response to vasopressin, AQP2 traffics from intracellular vesicles to the apical plasma membrane of collecting duct principal cells, where it increases water
Functional characterization of ion channels expressed in kidney organoids derived from human induced pluripotent stem cells
Montalbetti N, et al.
American Journal of Physiology: Renal Physiology, 323(4), F479-F491 (2022)
Resin-assisted enrichment of thiols as a general strategy for proteomic profiling of cysteine-based reversible modifications
Guo J, et al.
Nature Protocols, 9, 64-75 (2014)
Zhengxia Wang et al.
Allergy, asthma & immunology research, 12(5), 859-876 (2020-07-09)
CD4⁺T cells are essential in the pathogenesis of allergic asthma. We have previously demonstrated that microRNA-1165-3p (miR-1165-3p) was significantly reduced in T-helper type (Th) 2 cells and that miR-1165-3p was a surrogate marker for atopic asthma. Little is known about
Junyi Gao et al.
Journal of cellular and molecular medicine, 24(21), 12726-12735 (2020-10-08)
CDHR5 has been reported to play key roles in carcinogenesis of various cancers, but its roles in pancreatic cancer have not been reported. The present study was designed to investigate its clinical value in pancreatic ductal adenocarcinoma (PDAC). Tissue microarray-based
資料
Learn tips and tricks for how to properly use inhibitors including how to select the right inhibitor and how to plan experiments with inhibitors.
関連コンテンツ
An Introduction to Inhibitors and Their Biological Applications
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 539134-1SETCN | 07790788059750 |
| 539134-1MLCN | 07790788051280 |
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