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Merck

556881

Ribonuclease Inhibitor, Human, Recombinant, E. coli

The Ribonuclease Inhibitor, Human, Recombinant, E. coli controls the biological activity of Ribonuclease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

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この商品について

UNSPSC Code:
12352200
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form

liquid

specific activity

≥20,000 units/mL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

foreign activity

Endonuclease and RNase, none detected

shipped in

wet ice

storage temp.

−20°C

Quality Level

General description

Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes, and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.

Biochem/physiol Actions

Cell permeable: no
Primary Target
inactivates RNase
Product does not compete with ATP.
Reversible: no

Physical form

In 50 mM KCl, 20 mM HEPES, 8 mM DTT, 50% glycerol.

Preparation Note

Maintain at -20°C. Avoid freezing solutions. Addition of DTT is recommended to maintain 8 mM level.

Other Notes

One unit is defined as the amount of material that will inhibit 50% of the activity of 5 ng of RNase A.
Saxena, S.K., et al. 1992. J. Biol. Chem. 267, 21982.
Lee, F.S., et al. 1989. Biochemistry 28, 225.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

保管分類

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

556881-220KU: + 556881-U: + 556881-2500U: + 970918-220KU:

jan


試験成績書(COA)

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Asimina Katsiani et al.
The Journal of general virology, 101(6), 676-684 (2020-05-08)
Rose rosette virus (RRV) (genus Emaravirus) is the causal agent of the homonymous disease, the most destructive malady of roses in the USA. Although the importance of the disease is recognized, little sequence information and no full genomes are available
Vera Thole et al.
BMC genomics, 20(1), 995-995 (2019-12-21)
Flavonoids are produced in all flowering plants in a wide range of tissues including in berry fruits. These compounds are of considerable interest for their biological activities, health benefits and potential pharmacological applications. However, transcriptomic and genomic resources for wild
Nida Ali et al.
Nucleic acids research, 48(2), 847-861 (2019-12-06)
RNase E is a 472-kDa homo-tetrameric essential endoribonuclease involved in RNA processing and turnover in Escherichia coli. In its N-terminal half (NTH) is the catalytic active site, as also a substrate 5'-sensor pocket that renders enzyme activity maximal on 5'-monophosphorylated
Ihshan Akthar et al.
Reproduction (Cambridge, England) (2019-12-04)
We previously reported that sperm binding to cultured monolayers of bovine uterine epithelial cells induces an acute inflammatory response involving the Toll-like receptor (TLR2) signaling pathway. This response serves to clear the uterus of sperm and thereby prepares the endometrium
Claudia S Barros et al.
STAR protocols, 3(4), 101735-101735 (2022-10-02)
Here, we describe a protocol to remove single identified cells directly from Drosophila living brains and analyze their transcriptome. We detail the steps to harvest fluorescent cells using a capillary under epifluorescence and transmitted light to avoid contamination. We then

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