Anti-Vesicle protein sorting 35 (VPS35) Antibody

Vacuolar protein sorting-associated protein 35 (VPS35) is critical component found within the retromer complex. The retromer complex is comprised of several proteins and has been implicated in roles involving the Wnt signaling pathway, cell migration and adhesion, and polymeric immunoglobulin A transcytosis. VPS35 plays an essential role in the selection and trafficking of lysosomal enzyme receptors from endosomes to the Golgi. It is also a required component for the secretion of active Wnt. Expression of VPS35 is pervasive throughout all tissue. Highest levels of expression have been observed in brain, colon, heart, kidney, ovary, placenta, skeletal muscle, small intestine, spleen, testis, and thymus.

~ 80 kDa observed MW. Expected MW is 92 kDa

GST-tagged recombinant protein corresponding to human VPS35.

Anti-Vesicle protein sorting 35 (VPS35) Antibody is an antibody against Vesicle protein sorting 35 (VPS35) for use in WB.

RNAi: A previous lot of antibody was used in siRNA knockdown experiments by an independent laboratory (Courtesy of Gomez, TS, Mayo Clinic, 2010).

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Format: Purified

Protein G

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
A431 cell lysate

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected VPS35 in 10 µg of A431 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.