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Merck

ECM555

QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric

The CHEMICON Cell Invasion Assay Kit employs a 96-well plate, and provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.

別名:

8 μm pore cell invasion assay

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この商品について

UNSPSC Code:
12352207
NACRES:
NA.32
eCl@ss:
32161000

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製品名

QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric, The CHEMICON Cell Invasion Assay Kit employs a 96-well plate, and provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.

species reactivity

human

manufacturer/tradename

Chemicon®
QCM

technique(s)

activity assay: suitable
cell based assay: suitable

detection method

fluorometric

shipped in

wet ice

Quality Level

100

関連するカテゴリー

Application

QCM ECMatrix Cell Invasion Assay, 96-well (8 μm), fluorimetric has been used in the cell invasion assay of:

  • human cervical carcinoma
  • osteosarcoma
  • breast cancer cells
  • colorectal cancer cells
Ideal for screening pharmacological agents, evaluating MMPs or proteases responsible for cell invasion, and gene function analysis in transfected cells

Features and Benefits

  • The assay does not require cell labeling, scraping, washing, or counting
  • Suitable for large-scale screening and quantitative comparison of multiple samples due to homogenous fluorescence detection and 96 well insert
  • Enables a quick and efficient system for the quantitative determination of several factors for cell invasion

General description

QCM ECMatrix Cell Invasion Assay involves invaded cells at the bottom of the insert membrane and can be obtained by treating the membrane with cell detachment buffer. The cells are lysed and detected by the patented CyQuant GR dye. This dye is a green-fluorescent dye and binds well with cellular nucleic acids to emit a strong fluorescence. This assay kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.

Other Notes

Sterile 96-well Cell Invasion Plate Assembly: (Part No. 90200) One 96-well feeder tray, and one 96-well Cell Invasion Chamber plate with ECMatrixTM -coated inserts.

96-well Cell Culture Tray: (Part No. 90129) One 96-well feeder tray.

Cell Detachment Solution: (Part No. 90131) One bottle - 16 mL.

4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.

CyQuant GR Dye: (Part No. 90132) One vial - 75 μL

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

hcodes

H318,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

保管分類

10 - Combustible liquids

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

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prtr

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fsl

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ishl_indicated

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試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Sodium arsenite inhibits migration of extravillous trophoblast cells in vitro.
Chi-Shan Li,Rita Loch-Caruso
Reproductive Toxicology null
Increases in mitochondrial biogenesis impair carcinogenesis at multiple levels.
Xiao Wang,Carlos T Moraes
Molecular Oncology null
Sheeba Irshad et al.
Cancer research, 77(5), 1083-1096 (2017-01-14)
Cancer cells tend to metastasize first to tumor-draining lymph nodes, but the mechanisms mediating cancer cell invasion into the lymphatic vasculature remain little understood. Here, we show that in the human breast tumor microenvironment (TME), the presence of increased numbers
Targeting of Integrin {beta}1 and Kinesin 2{alpha} by MicroRNA 183.
Li G, Luna C, Qiu J, Epstein DL, Gonzalez P
The Journal of Biological Chemistry null
Differential expression of matrix metalloproteinases in brain- and bone-seeking clones of metastatic MDA-MB-231 breast cancer cells.
Andreas M Stark,Bartosz Anuszkiewicz,Rolf Mentlein,Toshiyuki Yoneda,H Maximilian Mehdorn et al.
Journal of Neuro-Oncology null
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関連コンテンツ

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Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression

"Recognizing both the tremendous opportunities and the challenges facing cancer research, we are dedicated to developing and refining tools and technologies for the study of cancer. With our comprehensive portfolio, including the Upstate®, Chemicon®, and Calbiochem® brands of reagents and antibodies, researchers can count on dependable, high quality solutions for analyzing all the hallmarks of cancer."

Cell Migration and Invasion: Choosing the Right Assay

Cell migration is stimulated and directed by interaction of cells with the extracellular matrix (ECM), neighboring cells, or chemoattractants. Cell migration participates in morphogenic processes, wound healing and tumor metastasis. Specifically, inhibiting tumor invasion by blocking tumor cell chemotaxis has been a major focus of research. Tumor cell invasion, marked by degradation of ECM, is also directly correlated with metastatic potential.

グローバルトレードアイテム番号

カタログ番号GTIN
ECM55504053252745188

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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