Anti-Presenilin-1 Antibody, loop, a.a. 263-378, CT, clone PS1-loop

18-20 kDa

Presenilin is a probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Presenilin requires the other members of the gamma-secretase complex to have a protease activity. It may play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. The gamma-secretase complex stimulates cell-cell adhesion through its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, it cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. Futher researcher may implicate the complex in hematopoiesis. Defects in PSEN1 have been implicated in early-onset Alzheimer disease type 3 (AD3) and late-onset Alzheimer disease (LOAD).

Epitope: C-terminus

Presenilin-1, loop fusion protein (amino acids 263-378), human

Anti-Presenilin-1 Antibody, loop, a.a. 263-378, C-terminus, clone PS1-loop detects level of Presenilin-1 & has been published & validated for use in IC, IH(P), IP & WB.

Immunocytochemistry:
A previous lot of this antibody was used in IC.Acetone or methanol fixed cells; or lightly fixed 2.5-3% PFA (5-10′ RT).

Immunohistochemistry:
A previous lot was used on 3% paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections if fixed with PLP, acetone or other light fixations; 10% formalin fixation is untested.

Immunoprecipitation:
A previous lot of this antibody was used in IP.

Western blot:
Recognizes a protein of approximately 18-20 kD, and occasionally there is a doublet (the top band of which is phosphorylated).

Optimal working dilutions must be determined by the end user

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Presenilin-1, loop

Mouse monoclonal IgG1 ascites with no preservatives.

Unpurified

Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Brain tissue

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected PRESENILIN-1 on 10 μg of PC12 lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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