NE1022
PhosphoDetect Anti-Neurofilament H Mouse mAb (SMI-31)
liquid, clone SMI-31, Calbiochem®
別名:
Anti-neurofilament antibody
製品名
PhosphoDetect Anti-Neurofilament H Mouse mAb (SMI-31), liquid, clone SMI-31, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
SMI-31, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, human, rat
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(ELISA (1:1000)
Frozen Sections (1:1000)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000)
Paraffin Sections (1:1000, heat pre-treatment required)
Immunoprecipitation )
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NEFH(4744)
Application
ELISA (1:1000)
Frozen Sections (1:1000, see comments)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000, see comments)
Paraffin Sections (1:1000, heat pre-treatment required, see comments)
Immunoprecipitation (see comments)
Disclaimer
Toxicity: Standard Handling (A)
General description
Mouse monoclonal antibody supplied as purified antibody. Recognizes the ~180-200 kDa phosphorylated neurofilament H protein.
Recognizes the ~180-200 kDa phosphorylated neurofilament H protein in rat central nervous system (CNS) cytoskeletal preparations. Also weakly recognizes phosphorylated NF-M protein.
This PhosphoDetect Anti-Neurofilament H Mouse mAb (SMI-31) is validated for use in ELISA, Frozen Sections, Immunoblotting, ICC, Paraffin Sections, IP for the detection of Neurofilament H.
Immunogen
homogenized, hypothalami from Fischer 344 rat brain
Other Notes
Raina, A.K., et al. 1999. Neuroreport10, 1355.
Yang, C.C., et al. 1998. Brain121, 1089.
Giasson, B.I and Mushynski, W.E. 1996. J. Biol. Chem.271, 30404.
Mirabella, M., et al. 1996. J. Neuropath. Exp. Neurol.55, 774.
Xiao, J. and Monteiro, M.J. 1994. J. Neurosci.14, 1820.
Yang, C.C., et al. 1998. Brain121, 1089.
Giasson, B.I and Mushynski, W.E. 1996. J. Biol. Chem.271, 30404.
Mirabella, M., et al. 1996. J. Neuropath. Exp. Neurol.55, 774.
Xiao, J. and Monteiro, M.J. 1994. J. Neurosci.14, 1820.
Strongly recognizes phosphorylated neurofilament H and, to a lesser extent, phosphorylated neurofilament M. By immunocytochemistry this antibody broadly stains thick and thin axons and some dendrites, such as basket cell dendrites, but not Purkinje cell dendrites. Does not generally stain nerve cell bodies or other cells and tissues except peripheral axons. May also stain neuronal cell bodies in pathological conditions. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures following treatment with agents that induce stress-activated protein kinase. This antibody is reported to co-immunoprecipitate neurofilament-associated kinase (NAK 115) via interaction of the antibody with the tail domain of neurofilament H. Phosphatase treatment of tissue sections or immunoblotting samples abolishes antibody reactivity. Reactivity is unaffected by trypsin treatment of samples. Tissues and cultured cells can be fixed with a variety of paraformaldehyde- or formaldehyde-containing fixatives, including Bouin′s fixative. Post-fixation in cold methanol or methanol/hydrogen peroxide facilitates access of the antibody to the epitope in frozen sections or thick tissue sections fixed in 4% paraformaldehyde and in cultured cells. For staining formalin-fixed, paraffin sections it is recommended that the de-paraffinized tissue be autoclaved in dH2O for 10 min or boiled in Tris-buffered saline, pH 9.0, in a microwave for 15 min to expose the epitope. Antibody should be titrated for optimal results in individual systems.
Physical form
Phosphate buffer solution, pH7.2, containing 0.09% sodium azide
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| NE1022-100ULCN | 04055977209907 |
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