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Merck

PC130

Anti-Rad51 (Ab-1) Rabbit pAb

liquid, Calbiochem®

別名:

Anti-Rad51, Rabbit Anti-Rad51, Rad51 Detection Antibody

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この商品について

NACRES:
NA.41
UNSPSC Code:
12352203

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製品名

Anti-Rad51 (Ab-1) Rabbit pAb, liquid, Calbiochem®

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, rat, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(Immunoblotting (1:2500)
Immunoprecipitation (40 µL/500µg total protein from lysate)
Immunocytochemistry (1:500))

isotype

IgG

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... RAD51(5888)

Analysis Note

Positive Control
A431 cells, HeLa cells

Application




Immunoblotting (1:2500)
Immunoprecipitation (40 l/500g total protein from lysate)
Immunocytochemistry (1:500)

Disclaimer

Toxicity: Standard Handling (A)

General description

Affinity purified rabbit polyclonal antibody. Recognizes the ~43 kDa Rad51 protein.
Recognizes the ~43 kDa Rad51 protein in ovarian tissue.
The bacterial RecA and the yeast RAD51 proteins are involved in homologous recombination and DNA repair. The human RAD51 protein is the homologue of these two proteins. While the modular structure of these proteins is conserved and the homology is very strong with the bacterial protein there are functional differences seen with RAD51 including an ATP hydrolysis rate 1/10 that seen with RecA. Although RAD51 is a nuclear protein and is largely absent from the cytoplasm and nucleoli, the protein lacks a nuclear localization signal and requires other proteins in a complex to be translocated into the nucleus. Treatment of cells with DNA synthesis inducing agents will cause an increase in the levels of RAD51 protein which then undergo a shift in distribution becoming focal in nature. This focal distribution may be blocked by inhibitors of transcription. RAD51 can be shown to interact directly with the chromosomes and during the process of meiosis, its distribution of RAD51 undergoes dynamic change. RAD51 has been shown to interact with a wide variety of different proteins that include the wild-type form of p53, BRCA2, and RAD52, which specifically modulates its activity.
This Anti-Rad51 (Ab-1) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of Rad51 (Ab-1).

Immunogen

Human
recombinant, human Rad51

Other Notes

Brendel, V., et al. 1997. J. Mol. Evol.44, 528.
Boulikas, T., et al. 1997. Anticancer Res.17, 843.
Gupta, R.C., et al. 1997. Proc. Natl. Acad. Sci. USA94, 463.
Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
Sharan, S.K., et al. 1997. Nature386, 304.
Li, M.J., et al. 1996. Proc. Natl. Acad. Sci. USA93, 10222.
Shen, Z., et al. 1996. J. Biol. Chem.271, 148.
Sturzbecher, H.W., et al. 1996. EMBO J. 15, 1992.
Tashiro, S., et al. 1996. Oncogene12, 2165.
Ashley, T., et al. 1995. Chromosoma104, 19.
Haaf, T., et al. 1995. Proc. Natl. Acad. Sci. USA92, 2298.
Due to a high degree of sequence identity, this antibody is also expected to cross-react with bovine, equine, and canine RAD51. Antibody should be titrated for individual systems.

Physical form

In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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保管分類

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Nuria Coll-Bonfill et al.
Proteomics, 20(5-6), e1800406-e1800406 (2019-12-14)
Hutchinson Gilford progeria syndrome (HGPS) is a devastating accelerated aging disease caused by LMNA gene mutation. The truncated lamin A protein produced "progerin" has a dominant toxic effect in cells, causing disruption of nuclear architecture and chromatin structure, genomic instability
Roberto de la Fuente et al.
PLoS genetics, 3(11), e198-e198 (2007-11-07)
In most eutherian mammals, sex chromosomes synapse and recombine during male meiosis in a small region called pseudoautosomal region. However in some species sex chromosomes do not synapse, and how these chromosomes manage to ensure their proper segregation is under
Karen J Ouyang et al.
PLoS biology, 7(12), e1000252-e1000252 (2009-12-04)
The gene mutated in Bloom's syndrome, BLM, is important in the repair of damaged replication forks, and it has both pro- and anti-recombinogenic roles in homologous recombination (HR). At damaged forks, BLM interacts with RAD51 recombinase, the essential enzyme in
Yue Sun et al.
Cell death & disease, 11(4), 292-292 (2020-04-29)
Genome instability is the fundamental hallmark of malignant tumors. Tumor suppressors often play a role in maintaining genome stability. Our previous genetic screen identified inositol polyphosphate 4-phosphatase type B (INPP4B), primarily hydrolyzing phosphatidylinositol 3, 4-disphosphate, is a potential tumor suppressor
Céline Jacquemont et al.
Molecular cancer, 11, 26-26 (2012-04-28)
Platinum compounds such as cisplatin and carboplatin are DNA crosslinking agents widely used for cancer chemotherapy. However, the effectiveness of platinum compounds is often tempered by the acquisition of cellular drug resistance. Until now, no pharmacological approach has successfully overcome
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グローバルトレードアイテム番号

カタログ番号GTIN
PC130-100ULCN07790788053949

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