ST1202
Anti-PGC-1α Mouse mAb (4C1.3)
liquid, clone 4C1.3, Calbiochem®
別名:
Anti-Peroxisome Proliferator-Activated Receptor- γ Coactivator 1 α
ログインで組織・契約価格をご覧ください。
サイズを選択してください
この商品について
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
4C1.3, monoclonal
Species reactivity:
mouse, human, rat
Application:
Citations:
154
製品名
Anti-PGC-1α Mouse mAb (4C1.3), liquid, clone 4C1.3, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
4C1.3, monoclonal
form
liquid
does not contain
preservative
species reactivity
mouse, human, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1 µg/mL)
Immunocytochemistry (5-10 µg/mL)
Immunoprecipitation (10 µg/mL)
Paraffin Sections (whole mount) )
isotype
IgG
storage temp.
−70°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PPARGC1A(10891)
Application
Immunoblotting (1 µg/ml)
Immunocytochemistry (5-10 µg/ml)
Immunoprecipitation (10 µg/ml)
Paraffin Sections (whole mount) (see application references)
Immunocytochemistry (5-10 µg/ml)
Immunoprecipitation (10 µg/ml)
Paraffin Sections (whole mount) (see application references)
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
General description
PGC-1α is a co-activator of PPARα, PPARγ, and other transcription factors and regulates the transcriptonal program of adaptive thermogenesis in brown adipose tissue, hepatic/renal gluconeogenesis, and muscle fiber type switching. The full-length protein is 113 kDa and is induced in brown adipose tissue by cold exposure, in liver and kidney by fasting, and in skeletal muscle by exercise. Alternative splicing of the full-length gene produces a 270 aa N-terminal splice variant that migrates at ~38 kDa and is induced by the same physiological signals that induce expression of the full-length protein. The N-terminal PGC-1α splice variant is also subject to post translational modifications that alter its migration and apparent molecular weight.
Protein G purified mouse monclonal antibody. Recognizes the endogenous forms of PGC-1α, which includes the ~113 kDa PGC-1α protein and the ~38 kDa splice variant.
Recognizes the endogenous forms of PGC-1α, which includes the ~113 kDa PGC-1α protein and the ~38 kDa splice variant, in brown adipose tissue, liver, and kidney.
This Anti-PGC-1α Mouse mAb (4C1.3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation, Paraffin Sections for the detection of PGC-1α.
Immunogen
Mouse
a recombinant protein consisting of amino acids 1-120 of mouse PGC-1α
Other Notes
Endogenous PGC-1α is found primarily in the nucleus and NT-PGC-1α is found in both cytosolic and nuclear fractions. Antibody should be titrated for optimal results in individual systems.
Lai, L., et al. 2008. Genes Dev. 14, 1948.
Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
Physical form
One vial of 100 µg antibody in 50 mM PBS, see vial for lot-specific concentration. One vial of NT-PGC-1α Positive Control, supplied as 25 µl whole cell extract in RIPA buffer containing 10 mM &beta-mercaptoethanol and 1% SDS; load 10 µl per lane; add sample buffer prior to SDS-PAGE loading.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類
10 - Combustible liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Please refer to KIT Component information
pdsc
Please refer to KIT Component information
prtr
Please refer to KIT Component information
fsl
Please refer to KIT Component information
ishl_indicated
Please refer to KIT Component information
ishl_notified
Please refer to KIT Component information
cart
キットコンポーネントの情報を参照してください
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Amy E Mendham et al.
Journal of applied physiology (Bethesda, Md. : 1985), 121(6), 1326-1334 (2016-10-16)
This study assessed the mitochondrial related signaling responses to a single bout of noncontact, modified football (touch rugby), played as small-sided games (SSG), or cycling (CYC) exercise in sedentary, obese, middle-aged men. In a randomized, crossover design, nine middle-aged, sedentary
Mariana Aguiar de Matos et al.
Frontiers in physiology, 9, 1451-1451 (2018-11-16)
Background: The excess body fat characteristic of obesity is related to various metabolic alterations, which includes insulin resistance (IR). Among the non-pharmacological measures used to improve insulin sensitivity are aerobic physical training, such as high-intensity interval training (HIIT). This study
Naoki Horii et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 312(4), R520-R528 (2017-01-27)
The purpose of this study was to investigate the effect of chronic chlorella intake alone or in combination with high-intensity intermittent exercise (HIIE) training on exercise performance and muscle glycolytic and oxidative metabolism in rats. Forty male Sprague-Dawley rats were
Tess V Dupre et al.
Biochemical pharmacology, 169, 113644-113644 (2019-09-23)
A hallmark of acute kidney injury (AKI) is vascular rarefication and mitochondrial dysfunction. Promoting vascular recovery following AKI could facilitate kidney repair as the vasculature is responsible for oxygen and nutrient delivery to extravascular tissues. Little is known about mitochondrial
Sudipta Chakrabarti et al.
Neurobiology of disease, 127, 362-373 (2019-04-01)
The late-infantile Batten disease or late-infantile neuronal ceroid lipofuscinosis (LINCL) is an autosomal recessive lysosomal storage disorder caused by mutations in the Cln2 gene leading to deficiency of lysosomal enzyme tripeptidyl peptidase 1 (TPP1). At present, available options for this
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）