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Merck

UFC8100

Amicon® Ultra Centrifugal Filter, 100 kDa MWCO

greener alternative

sample volume 4 mL, regenerated cellulose membrane, MWCO 100 kDa

別名:

Centrifugal concentrator,100 kDa MWCO, 4 mL sample volume

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この商品について

UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing
Pore size:
100 kDa MWCO
Working volume:
≤4 mL , 4 mL
Sterility:
non-sterile
Packaging:
pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg

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Quality Level

400

material

polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing

sterility

non-sterile

product line

Amicon®

packaging

pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg

manufacturer/tradename

Amicon® Ultra

sustainability

Greener Alternative Product

parameter

4 mL sample volume

technique(s)

protein extraction: suitable, protein purification: suitable

L

124 mm (4.88 in.)

diam.

17.3 mm

filtration area

3 cm2

volume

4 mL

working volume

≤4 mL , 4 mL

pore size

100 kDa MWCO

greener alternative category

Re-engineered

shipped in

ambient

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関連するカテゴリー

General description

Amicon® Ultra 4 mL centrifugal devices with Ultracel® 100,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, IgG (156 kDa) at 1 mg/ml initial concentration is retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.

Application

  • Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
  • Concentration of dilute or pre-purified proteins from column eluents.
  • Desalting, buffer exchange and protein dialysis.
  • Size exclusion and protein fractionation.

Features and Benefits

  • Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%.
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
  • Heat-sealed membrane minimizes downstream extractables.
  • 100% integrity tested for reliable performance.
  • Convenient sample monitoring with translucent housing and volume gradations.
  • Direct pipette sample access eliminates processing step to recover concentrate.
  • High concentration factors of 80–100X.

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

保管分類

11 - Combustible Solids

試験成績書（COA）

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資料

ナノ粒子の限外ろ過

ナノ粒子の精製と製造における、ろ過法とメンブレンフィルターの選定。

タンパク質サンプルの限外ろ過

溶媒交換やサンプル濃縮などのタンパク質サンプル調製のための限外ろ過膜の原理と利点をご覧ください。

Protein Sample Ultrafiltration

Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.

Nanoparticle Ultrafiltration

Filtration methods and membrane filter selection in nanoparticle purification and production.

プロトコル

限外ろ過よる簡単なタンパク質濃縮法

ヒト血漿プロテオームは、バイオマーカーの発見と検討を行うための豊富な資源です。

A Simple Strategy for Protein Enrichment Using Ultrafiltration

The human plasma proteome is a vast resource for biomarker discovery and investigation.

グローバルトレードアイテム番号

カタログ番号GTIN
UFC81002404053252666377
UFC81000804053252612169
UFC81009604053252726576

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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