94072
Phalloidin–Atto 565
suitable for fluorescence, ≥80.0% (HPLC)
assay
≥80.0% (HPLC)
form
solid
mol wt
Mw 1394 g/mol
manufacturer/tradename
ATTO-TEC GmbH
λ
in methanol
UV absorption
λ: 562.0-568.0 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
Quality Level
General description
Atto 565 is a novel fluorescent label that belongs to the class of Rhodamine dyes. It shows a strong absorption, high fluorescence quantum yield, high thermal and photostability, and a very little triplet formation. Atto 565 consists of a mixture of two isomers with practically identical optical absorption and emission Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10-8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours. Fluorescent conjugates of phalloidin are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-d-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.
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保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
94072-10NMOL:
jan
Recombinant Extracellular Matrix Protein Fragments Support Human Embryonic Stem Cell Chondrogenesis.
Aixin Cheng et al.
Tissue engineering. Part A, 24(11-12), 968-978 (2017-12-28)
We previously developed a 14-day culture protocol under potentially GMP, chemically defined conditions, to generate chondroprogenitors from human embryonic stem cells (hESCs). In vivo work has confirmed the cartilage repair capacity of these cells in a nude rat osteochondral defect
Mariangela Sabatella et al.
Cell reports, 34(2), 108608-108608 (2021-01-14)
Hereditary DNA repair defects affect tissues differently, suggesting that in vivo cells respond differently to DNA damage. Knowledge of the DNA damage response, however, is largely based on in vitro and cell culture studies, and it is currently unclear whether DNA repair
Thomas Bise et al.
Scientific reports, 10(1), 11551-11551 (2020-07-16)
Zebrafish can regenerate their damaged hearts throughout their lifespan. It is, however, unknown, whether regeneration remains effective when challenged with successive cycles of cardiac damage in the same animals. Here, we assessed ventricular restoration after two, three and six cryoinjuries
Silke Rothenbusch-Fender et al.
Biology open, 6(12), 1876-1888 (2017-11-11)
During
Preethi P et al.
Frontiers in immunology, 11, 1528-1528 (2020-08-28)
A large body of research implicates the brain and fat body (liver equivalent) as central players in coordinating growth and nutritional homeostasis in multicellular animals. In this regard, an underlying connection between immune cells and growth is also evident, although
資料
Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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