GSK3β、活性型、Hisタグ融合 ヒト

Research area: Cellsignalling. GSK3B (glycogen synthase kinase 3β) is one of the two isoforms of GSK3 enzyme, both of which are encoded by different genes. This protein is predominantly cytoplasmic, but is also found to be localized to nucleus and mitochondria. It is a protein-serine kinase. This protein contains a protein kinase domain in its core, and is composed of 482 amino acids with a molecular weight of 46,712Da. It shows a wide range of tissue expression with the highest expression in brain.

GSK3β, active, His tagged human has been used as an incubating medium during in vitro hyperphosphorylation of tau protein.

GSK3βは、もともとはグリコーゲン合成酵素をリン酸化し、阻害するキナーゼとして同定されたセリンスレオニンプロテインキナーゼです。ヒト組織に広範に存在し、インスリンによるグリコーゲンおよびタンパク質合成の調節、転写因子AP-1およびCREBの調節など、いくつかの生理的過程の調節に関連づけられています。また、ヒトタウの各アイソフォームを安定トランスフェクションしたチャイニーズハムスター卵巣細胞でヒトGSK3βを一過性トランスフェクションした際に、リン酸化型タウの抗体を用いて分析されたすべての部位でタウの過剰リン酸化が確認されています。

Glycogen Synthase Kinase 3β (GSK3β) is responsible for rapid cyclin D1 turnover by phosphorylating it on Thr-286. It functions as a negative regulator of Wnt signaling during embryonic development and cell proliferation in adult tissues. This protein phosphorylates and regulates multiple proteins, such as glycogen synthase, insulin receptor substrate-1, axin and APC (adenomatous polyposis coli). This protein is also shown to interact with multiple proteins which are linked with Alzheimer′s disease (AD).

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