A0224
Anti-l/s-Afadin antibody produced in rabbit
affinity isolated antibody
別名:
Anti-AF6, Anti-MLL-AF6, Anti-MLLT4, Anti-l-afadin
ログインで組織・契約価格をご覧ください。
サイズを選択してください
この商品について
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunocytochemistry
immunohistochemistry (frozen sections)
indirect immunofluorescence
indirect immunofluorescence
microarray
western blot
immunohistochemistry (frozen sections)
indirect immunofluorescence
indirect immunofluorescence
microarray
western blot
Species reactivity:
rat, human, canine, mouse
Citations:
29
Technique(s):
immunocytochemistry: suitable
immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections.
indirect immunofluorescence: 1:250 using cultured dog (MDCK) cells and of cultured human HepG2 cells
indirect immunofluorescence: 1:500 using frozen sections of mouse liver
microarray: suitable
western blot: 1:1000 using extract of rat brain.
immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections.
indirect immunofluorescence: 1:250 using cultured dog (MDCK) cells and of cultured human HepG2 cells
indirect immunofluorescence: 1:500 using frozen sections of mouse liver
microarray: suitable
western blot: 1:1000 using extract of rat brain.
製品名
Anti-l/s-Afadin antibody produced in rabbit, affinity isolated antibody
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
mol wt
antigen ~200 kDa (and 190 kDa)
species reactivity
rat, human, canine, mouse
enhanced validation
independent
Learn more about Antibody Enhanced Validation
technique(s)
immunocytochemistry: suitable
immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections.
indirect immunofluorescence: 1:250 using cultured dog (MDCK) cells and of cultured human HepG2 cells
indirect immunofluorescence: 1:500 using frozen sections of mouse liver
microarray: suitable
western blot: 1:1000 using extract of rat brain.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MLLT4(4301)
mouse ... Mllt4(17356)
rat ... Mllt4(26955)
Application
Anti-l/s-Afadin antibody produced in rabbit has been used in :
- immunocytochemistry
- immunohistochemistry
- immunoprecipitation
Biochem/physiol Actions
Anti-l/s-Afadin specifically binds to l-afadin and s-afadin by immunoblotting (approximately 200 and 190 kDa respectively). Additional bands may appear in certain extract preparations in western blot assays. The immunizing peptide specifically inhibits the staining of the I/s-afadin bands. The antibody is specific for human, dog, rat, and mouse l/s-afadin.
s-Afadin binds to several cytoplasmic proteins such as the small guanosine triphosphatase (GTPase) Ras, the tight junction protein ZO-1 2 and the vinculin binding protein ponsin/ sorbin and SH3 domain containing 1 (SH3P12). In addition, l-afadin also binds through its PDZ several integral membrane components such as the Ca2+ -independent homophilic immunoglobulin-like nectin, the junctional adhesion molecule (JAM)4 and a subset of the EphB receptor protein-tyrosine kinases.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Afadin is a cell-cell adherens junction, F-actin binding multidomain protein. This adherens junction protein has two splicing variants: the ubiquitously expressed l-afadin, and the smaller s-afadin, which is abundantly expressed in neural tissues. Afadin associates with E-catenin proteins.
S-Afadin is a protein which lacks both the third proline-rich and the F-actin binding regions and is homologous to the human AF-6 (ALL-1 fusion partner from chromosome 6) gene product. Anti-l/s-Afadin is developed in rabbit using a synthetic peptide of rat afadin.
Immunogen
synthetic peptide corresponding to rat l/s afadin sequence, amino acids 1163-1179, with N-terminal cysteine added, conjugated to BSA.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
A0224-.2ML: + A0224-VAR: + IXO11335: + A0224-BULK:
jan
Afadin: A novel actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adherens junction
Mandai K, et al.
The Journal of cell biology, 139(2), 517-528 (1997)
Afadin is required for maintenance of dendritic structure and excitatory tone
Srivastava DP, et al.
The Journal of Biological Chemistry, 287(43), 35964-35974 (2012)
Morten Lundh et al.
Scientific reports, 11(1), 9794-9794 (2021-05-09)
The profound energy-expending nature of brown adipose tissue (BAT) thermogenesis makes it an attractive target tissue to combat obesity-associated metabolic disorders. While cold exposure is the strongest inducer of BAT activity, the temporal mechanisms tuning BAT adaptation during this activation
The junction-associated protein AF-6 interacts and clusters with specific Eph receptor tyrosine kinases at specialized sites of cell-cell contact in the brain
Buchert M, et al.
The Journal of cell biology, 144(2), 361-371 (1999)
Lei Gao et al.
Development (Cambridge, England), 144(19), 3511-3520 (2017-09-02)
In many types of tubules, continuity of the lumen is paramount to tubular function, yet how tubules generate lumen continuity in vivo is not known. We recently found that the F-actin-binding protein afadin is required for lumen continuity in developing
関連コンテンツ
Datasheet
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）