A2859
Monoclonal Anti-ATG5 antibody produced in mouse
clone ATG5-18, purified immunoglobulin, buffered aqueous solution
別名:
Anti-APG5, Anti-APG5L, Anti-ASP, Anti-ATG5 autophagy related 5 homolog, Anti-hAPG5
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この商品について
Conjugate:
unconjugated
Clone:
ATG5-18, monoclonal
Application:
IP, WB
Species reactivity:
human, mouse, rat
Citations:
34
Technique(s):
immunoprecipitation (IP): suitable, western blot: 2.5-5.0 μg/mL using whole extract of human K562 cells
Uniprot accession no.:
isotype
IgG2a
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ATG5-18, monoclonal
form
buffered aqueous solution
mol wt
antigen ~56 kDa
species reactivity
human, mouse, rat
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): suitable, western blot: 2.5-5.0 μg/mL using whole extract of human K562 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ATG5(9474)
mouse ... Atg5(11793)
rat ... Atg5(365601)
Application
Monoclonal Anti-ATG5 antibody produced in mouse has been used in western blotting and immunoprecipitation
Omegasomes were shown to localize to autophagosomes by immunohistochemistry using rabbit anti-ATG5 antibody.
Biochem/physiol Actions
Atg5 (also known as Apg5) is a 32kDa protein that is required for mediating autophagy. Studies have reported that the Atg12-Atg5-Atg16 complex localizes to autophagosome precursors and plays an important role in autophagosome formation. Furthermore, a fragment of Atg5 fragment produced by calpain cleavage is known to have pro-apoptotic properties.
Autophagy related 5 (ATG5), also known as Apg5, is essential for autophagy, a major pathway for bulk degradation of cytoplasmic constituents and organelles. In this process, portions of the cytoplasm are sequestered into double membrane vesicles, the autophagosomes, and subsequently delivered to the lysosome for degradation and recycling. Autophagy is required for normal turnover of cellular components during starvation. It plays an essential role in cellular differentiation, cell death and aging. Defective autophagy may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections. Autophagy is an evolutionary conserved pathway seen in all eukaryotic cells.
Mouse monoclonal anti-ATG5 antibody reacts with human, rat, and mouse Atg5-Atg12 complex by immunoblotting (∼56kDa) and immunoprecipitation.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-ATG5 (mouse IgG2a isotype) is derived from the hybridoma ATG5-18 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human Atg5, conjugated to keyhole limpet hemocyanin (KLH). The autophagy related 5 (ATG5) gene is mapped to human chromosome 6q21 and encodes a 32 kDa protein.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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保管分類
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
A2859-200UL: + A2859-100UL: + A2859-BULK: + A2859-25UL: + A2859-VAR:
jan
Xian Jiang et al.
Molecular cell, 63(2), 229-239 (2016-07-23)
In response to apoptotic stimuli, mitochondria in mammalian cells release cytochrome c and other apoptogenic proteins, leading to the subsequent activation of caspases and apoptotic cell death. This process is promoted by the pro-apoptotic members of the Bcl-2 family of
Mireia Niso-Santano et al.
The EMBO journal, 34(8), 1025-1041 (2015-01-15)
To obtain mechanistic insights into the cross talk between lipolysis and autophagy, two key metabolic responses to starvation, we screened the autophagy-inducing potential of a panel of fatty acids in human cancer cells. Both saturated and unsaturated fatty acids such
Allan Sauvat et al.
EBioMedicine, 30, 261-272 (2018-04-03)
Depending on the length of their carbon backbone and their saturation status, natural fatty acids have rather distinct biological effects. Thus, longevity of model organisms is increased by extra supply of the most abundant natural cis-unsaturated fatty acid, oleic acid
Atg5: more than an autophagy factor.
Patrice Codogno et al.
Nature cell biology, 8(10), 1045-1047 (2006-10-03)
Autophagy in health and disease: a double-edged sword
Shintani T and Klionsky DJ
Science (New York, N.Y.), 306(5698), 990-995 (2004)
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Instructions
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