A5230
モノクローナル抗ARF6抗体 マウス宿主抗体
clone ARFAG, purified from hybridoma cell culture
別名:
抗ADP-リボシル化因子6抗体
ログインで組織・契約価格をご覧ください。
サイズを選択してください
この商品について
Conjugate:
unconjugated
Clone:
ARFAG, monoclonal
Application:
WB
Citations:
13
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ARFAG, monoclonal
form
buffered aqueous solution
mol wt
antigen 18 kDa
species reactivity
bovine, canine, rat, human
concentration
2 mg/mL
technique(s)
western blot: 1-2 μg/mL using MDCK total cell extract.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ARF6(382)
rat ... Arf6(79121)
関連するカテゴリー
General description
ARF6 is a member of the ADP-ribosylation factor (ARF) family. It is specifically involved in endocytosis of plasma membrane proteins.
Anti-ARF6 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma ARFAG produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide. class III includes only human Arf6. The ADP-ribosylation factors (Arf) family of proteins, which belong to the Ras (rat sarcoma viral oncogene homolog) family, are low molecular weight proteins capable of binding GTP.
Application
Anti-ARF6 antibody, Mouse monoclonal has been used in:
- western blotting
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
Mouse Monoclonal Anti-ARF6 antibody can be used for western blot assays at 1-2μg/ml using MDCK cell extracts. The antibody can also be used for ELISA and immunocytochemistry.
Biochem/physiol Actions
ADP-ribosylation factor 6 (Arf6) is present at the plasma membrane and on endosomal membranes where it regulates the flow of trafficking into and out of the cell, and regulates the actin cytoskeleton on the plasma membrane. ARF6 activates phospholipase D (PLD) and phosphatidylinositol 4-phosphate 5-kinase (PIP5K) to generate phosphatidic acid and PIP2 (phosphotidylinositol 4,5-bisphosphate), respectively.
Physical form
0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
A5230-200UL: + A5230-VAR: + A5230-BULK:
jan
Summer F Acevedo et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(46), 15396-15402 (2015-11-21)
Alcohol use disorders (AUDs) affect people at great individual and societal cost. Individuals at risk for AUDs are sensitive to alcohol's rewarding effects and/or resistant to its aversive and sedating effects. The molecular basis for these traits is poorly understood.
D A Gonzalez et al.
Molecular psychiatry, 23(3), 621-628 (2017-06-14)
Ubiquitously expressed genes have been implicated in a variety of specific behaviors, including responses to ethanol. However, the mechanisms that confer this behavioral specificity have remained elusive. Previously, we showed that the ubiquitously expressed small GTPase Arf6 is required for
Multiple Roles for Arf6: Sorting, Structuring, and Signaling at the Plasma Membrane
Donaldson JG
The Journal of biological chemistry, 278(43), 41573-41576 (2003)
The Arf6 activator Efa6/PSD3 confers regional specificity and modulates ethanol consumption in Drosophila and humans
Gonzalez DA, et al.
Molecular Psychiatry, 23(3), 621-621 (2018)
Arf6 regulates the cycling and the readily releasable pool of synaptic vesicles at hippocampal synapse
Tagliatti E, et al.
eLife, 5, e10116-e10116 (2016)
関連コンテンツ
Instructions
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）