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この商品について
Form:
powder
Assay:
≥80% protein basis (biuret)
Recombinant:
expressed in corn
Mol wt:
glycoprotein 66 kDa, subunit 16 kDa
recombinant
expressed in corn
assay
≥80% protein basis (biuret)
form
powder
specific activity
≥12 units/mg protein (E1%/280)
mol wt
glycoprotein 66 kDa, subunit 16 kDa
technique(s)
ELISA: suitable, immunoblotting: suitable, immunoelectrophoresis: suitable
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
chicken ... AVD(396260)
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General description
Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.
Application
アビジン卵白は、ビオチン-アビジン結合を検出するために官能化キセノンをバイオセンサーとして使用するアッセイで使用されました。卵白は80nmolで使用されました。
Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.
Preparation Note
アフィニティ-精製済み
Other Notes
1 unitは、1.0 μgのd-ビオチンと結合する量です。
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
A8706-VAR: + A8706-5MG: + A8706-BULK:
jan
Sofia H L Frost et al.
Cancer biotherapy & radiopharmaceuticals, 28(2), 108-114 (2012-12-13)
Abstract Purpose: Pretargeted radioimmunotherapy (PRIT) against intraperitoneal (i.p.) ovarian microtumors using avidin-conjugated monoclonal antibody MX35 (avidin-MX35) and (211)At-labeled, biotinylated, succinylated poly-l-lysine ((211)At-B-PLsuc) was compared with conventional radioimmunotherapy (RIT) using (211)At-labeled MX35 in a nude mouse model. Mice were inoculated i.p.
Commercial Plant-Produced Recombinant Protein Products, 15-25 (2014)
Stefan Schillberg et al.
Journal of plant physiology, 258-259, 153359-153359 (2021-01-19)
Recombinant proteins play an important role in many areas of our lives. For example, recombinant enzymes are used in the food and chemical industries and as high-quality proteins for research, diagnostic and therapeutic applications. The production of recombinant proteins is
The avidin-biotin complex (ABC) method and other avidin-biotin binding methods.
G L Bratthauer
Methods in molecular biology (Clifton, N.J.), 115, 203-214 (1999-03-31)
Biopharmaceuticals in Plants: Toward the Next Century of Medicine, 121-122 (2009)
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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