C0733
シクロオキシゲナーゼ1 ヒツジ由来
glycerol solution, ≥1500 units/mg protein
別名:
COX-1, プロスタグランジンHシンターゼ1, プロスタグランジンエンドペルオキシドシンターゼ, 構成型シクロオキシゲナーゼ
製品名
シクロオキシゲナーゼ1 ヒツジ由来, glycerol solution, ≥1500 units/mg protein
form
glycerol solution
specific activity
≥1500 units/mg protein
mol wt
dimer subunit mol wt 70 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Quality Level
Gene Information
sheep ... COX1(808251)
Biochem/physiol Actions
COX-1はアラキドン酸をプロスタグランジンH2に変換するという、プロスタグランジン、トロンボキサン、プロスタサイクリンの生合成の第1段階の反応を触媒します。エイコサノイドの恒常性維持に関与しており、ほぼ全ての動物組織において構成的に発現しています。アラキドン酸に対する見かけのKMは8.3μMです。
Other Notes
1ユニットは、37°C、1分間で、100 μMアラキドン酸、5 mM EDTA、2 mMフェノール、1 μMヘマチンを含有する0.1 M Tris-HClバッファー(pH 8)中で1 nmolの酸素を消費する酵素量です。
Physical form
80mM Tris-HCl溶液 (pH 8, 0.1%TWEEN® 20, 300μMジエチルジチオカルバメートを含有)
Application
Cyclooxygenase 1 from sheep has been used as positive control protein in western blot analysis of osteoarthritis samples and in prostaglandin synthase activity assay.
General description
Cyclooxygenase 1 is a 71 kDa membrane bound protein predominantly present in endoplasmic reticulum. It has three domains, the epidermal growth factor (EGF) like domain, enzymatic and membrane binding domain. Cyclooxygenase 1 mediates prostaglandin synthesis and is modulated by anti inflammatory nonsteroidal drugs.
Legal Information
TWEEN is a registered trademark of Croda International PLC
保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Cyclo-oxygenase isoenzymes: physiological and pharmacological role
Kam PCA and See AUL
Anaesthesia, 55(5), 442-449 (2000)
Maurizio Anzini et al.
Journal of medicinal chemistry, 56(8), 3191-3206 (2013-03-29)
A series of 3-substituted 1,5-diarylpyrroles bearing a nitrooxyalkyl side chain linked to different spacers were designed. New classes of pyrrole-derived nitrooxyalkyl inverse esters, carbonates, and ethers (7-10) as COX-2 selective inhibitors and NO donors were synthesized and are herein reported.
The Sigma class glutathione transferase from the liver fluke Fasciola hepatica
LaCourse EJ, et al.
PLoS Neglected Tropical Diseases, 6(5), e1666-e1666 (2012)
S Ohki et al.
The Journal of biological chemistry, 254(3), 829-836 (1979-02-10)
The highly purified prostaglandin endoperoxide synthetase from bovine vesicular gland microsomes had two still unresolved enzyme activities; the oxygenative cyclization of 8,11,14-eicosatrienoic acid to produce prostaglandin G1 and the conversion of the 15-hydro-peroxide of prostaglandin G1 to a 15-hydroxyl group
Michela Zago et al.
Toxicology letters, 226(2), 107-116 (2014-01-30)
Diseases due to cigarette smoke exposure, including chronic obstructive pulmonary disease (COPD) and lung cancer, are associated with chronic inflammation typified by the increased expression of cyclooxygenase-2 (COX-2) protein. RelB is an NF-κB family member that suppresses cigarette smoke induction
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）