C3117
モノクロナール抗Cy3/Cy5
~1 mg/mL, clone CY-96, purified immunoglobulin, buffered aqueous solution
別名:
Monoclonal Anti-Cy3/Cy5
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この商品について
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
CY-96, monoclonal
Application:
ARR, DB, ELISA (d), ICC
Citations:
13
biological source
mouse
conjugate
biotin conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
CY-96, monoclonal
form
buffered aqueous solution
concentration
~1 mg/mL
technique(s)
direct ELISA: suitable, dot blot: suitable, immunocytochemistry: 1-2 μg/mL using chicken fibroblasts, microarray: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
関連するカテゴリー
General description
Monoclonal Anti-Cy3/Cy5 (mouse IgG2a isotype), Biotin conjugate is derived from the CY-96 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a mixture of proteins labelled with cyanine 3 (Cy3) or Cy5. Cyanine dyes are ionized polymethine dyes.
Immunogen
Cy3またはCy5標識タンパク質の混合物。
Application
Monoclonal Anti-Cy3/Cy5 Biotin antibody produced in mouse has also been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
- dot blot assay
- protein microarrays
- immunofluorescenc
Monoclonal Anti-Cy3/Cy5-Biotin antibody produced in mouse was used to label miR-107 in human breast cancer cell lines by confocal microscopy.
Biochem/physiol Actions
抗体は、Cy3, Cy5, AlexaFluro 647, AlexaFluor 547標識されたタンパク質を認識します。
Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.
Cyanine dyes are small, soluble in aqueous solution and has tolerance to dimethyl sulfoxide (DMSO). They are more photostable than fluorescein, has high molar extinction coefficients and favourable quantum yields. Monoclonal antibodies to cyanine 3 (Cy3) and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
C3117-VAR: + C3117-200UL: + C3117-BULK:
jan
Yejun Tan et al.
Frontiers in immunology, 14, 1149810-1149810 (2023-04-11)
Patients with diabetes mellitus (DM) have a higher incidence of malignant tumors than people without diabetes, but the underlying molecular mechanisms are still unclear. To investigate the link between DM and cancer, we screened publicly available databases for diabetes and
Chao-Yue Yang et al.
Chinese journal of traumatology = Zhonghua chuang shang za zhi, 26(3), 155-161 (2023-04-06)
This study aims to elucidate the electrotaxis response of alveolar epithelial cells (AECs) in direct-current electric fields (EFs), explore the impact of EFs on the cell fate of AECs, and lay the foundation for future exploitation of EFs for the
Wei Li et al.
Animal models and experimental medicine (2023-08-11)
The effect of platelet factor 4 (PF4) on bone marrow mesenchymal stem cells (BMMSCs) and osteoporosis is poorly understood. Therefore, this study aimed to evaluate the effects of PF4-triggered bone destruction in mice and determine the underlying mechanism. First, in
Quantitative profile of the uropathogenic Escherichia coli outer membrane proteome during growth in human urine
Alteri CJ and Mobley HLT
Infection and Immunity, 75(6), 2679-2688 (2007)
Pai-Sheng Chen et al.
The Journal of clinical investigation, 121(9), 3442-3455 (2011-08-16)
MicroRNAs (miRNAs) influence many biological processes, including cancer. They do so by posttranscriptionally repressing target mRNAs to which they have sequence complementarity. Although it has been postulated that miRNAs can regulate other miRNAs, this has never been shown experimentally to
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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