C5838
モノクロナール抗アクチン–Cy3 マウス宿主抗体
clone AC-40, purified immunoglobulin, buffered aqueous solution
別名:
モノクロナール抗アクチン
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この商品について
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
CY3 conjugate
Clone:
AC-40, monoclonal
Application:
direct immunofluorescence
Species reactivity:
human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat
Citations:
7
Technique(s):
direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts
製品名
モノクロナール抗アクチン–Cy3 マウス宿主抗体, clone AC-40, purified immunoglobulin, buffered aqueous solution
biological source
mouse
conjugate
CY3 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
AC-40, monoclonal
form
buffered aqueous solution
mol wt
antigen 42 kDa
species reactivity
human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat
technique(s)
direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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関連するカテゴリー
Application
モノクローナル抗アクチン抗体が、Cos-7細胞溶解物のウエスタンブロット解析において、タンパク質負荷量を等しくするために使用されました。
モノクローナル抗アクチン抗体を一次抗体として、MDCK細胞溶解物のウエスタンブロット解析が行われました。
Monoclonal Anti-Actin-Cy3 antibody has been used in immunofluorescent staining.
General description
抗体は、全てのアクチンアイソフォームで保存されている、アクチンのC末端に位置するエピトープを認識します。様々な組織及び種のアクチンを特異的に標識します。 抗体によって認識されるエピトープは、ホルマリン固定とパラフィン包埋に抵抗性を示します。亜鉛-ホルマリン、B5、エタノール、methacarn、Brunnel′又はBouin′液も、固定液として使用されます。
Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin has six isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three α-actins (α-skeletal, α-cardiac and α-smooth muscle), one β-actin (β-nonmuscle) and two γ-actins (γ-smooth muscle and γ-nonmuscle).
Immunogen
多抗原ペプチド（MAP）骨格に付着した合成アクチンC末端ペプチド
Legal Information
Amersham Biosciences Limited.からライセンスを受けて提供しています。
Physical form
0.01M PBS溶液(pH 7.4, 1%BSA, 15mMアジ化ナトリウム(防腐剤)含有)
Biochem/physiol Actions
Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Other Notes
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
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保管分類
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.
Gunning P, et al.
Molecular and Cellular Biology, 3(5), 787-795 (1983)
Erin Faught et al.
Scientific reports, 8(1), 18081-18081 (2018-12-29)
The mineralocorticoid receptor (MR) in mammals mediates the effects of aldosterone in regulating fluid balance and potassium homeostasis. While MR signalling is essential for survival in mammals, there is no evidence that MR has any physiological role in ray-finned fish.
Xiao-Yan Wen et al.
JCI insight, 3(24) (2018-12-21)
Sialic acids are important components of glycoproteins and glycolipids essential for cellular communication, infection, and metastasis. The importance of sialic acid biosynthesis in human physiology is well illustrated by the severe metabolic disorders in this pathway. However, the biological role
A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
Skalli O, et al.
The Journal of Cell Biology, 103(6), 2787-2796 (1986)
Wentao Shi et al.
Molecular medicine reports, 20(3), 2763-2773 (2019-07-20)
Ectomesenchymal stem cells (EMSCs) represent a type of adult stem cells derived from the cranial neural crest. These cells are capable of self‑renewal and have the potential for multidirectional differentiation. Tissue transglutaminase type 2 (TG2) is a ubiquitously expressed member
関連コンテンツ
Datasheet
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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