モノクロナール抗VSV 糖タンパク質−Cy3^™ マウス宿主抗体

Monoclonal Anti-VSV Glycoprotein (mouse IgG1 isotype) is derived from the P5D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide of vesicular stomatitis virus glycoprotein (VSV-G), conjugated to keyhole limpet hemocyanin (KLH). VSV-G is a protein expressed on the surface of bullet shaped virions.

KLHと結合した、水疱性口内炎ウイルス糖タンパク質(VSV-G)のC末端アミノ酸15残基(497~511)を含有する合成ペプチドのKLH結合体。

A dilution of 1:1000 of Monoclonal Anti-VSV Glycoprotein-Cy3^™ antibody produced in mouse was used to detect VSV glycoprotein in pig sperms by immunofluorescence.

Monoclonal Anti-VSV Glycoprotein Cy3^™ antibody produced in mouse has been used:

• in studies applying
• microinjection of antibody
• immunoblotting
• immunoprecipitation
• immunocytochemistry
• immunoelectron microscopy.

VSV糖タンパク質のC末端の5アミノ酸を含有するエピト-プを認識します。感染細胞において、細胞表面・出芽ウイルスに存在する成熟型VSV-Gおよび、粗面小胞体(RER)・ゴルジ複合体偏平嚢に存在する未成熟型VSV-Gも検出します。膜および細胞質ドメインを欠損したVSV-Gの分泌型とは反応しません。新規に合成されたVSV-Gが原形質膜・細胞表面に移動する間の細胞質ドメインの働きに関する研究における、マイクロインジェクション, イムノブロッティング, 免疫沈降法, 免疫細胞化学, 免疫電子顕微鏡法などに本抗体が用いられました。本抗体は、細胞外に発現するVSV-GのC末端でタグ標識した構造の検出, 免疫沈降, 免疫細胞化学的染色するのに用いられます。このタグは目的タンパク質の機能に干渉しません。また、各種の内因性タンパク質と交差反応のない、特異的なP5D4抗体で検出できます。

The envelope of vesicular stomatitis virus (VSV) consists of a bilayer membrane with a single type of glycoprotein, the G-protein (VSV-G) which mediates attachment to the cell surface and induces pH-dependent fusion between viral and target membranes. The carboxyl terminus of the VSV-G protein which does not have any homology with cellular proteins, has been engineered into expression vectors as a tag. Proteins expressed with this tag may thus be detected and localized using an antibody reactive specifically against this epitope with no risk of cellular background staining.

The vesicular stomatitis virus (VSV) glycoprotein facilitates the entry of HIV into the CD4 T cells through endocytotic pathway. The coupling of Cy3 to Anti-VSV Glycoprotein antibody allows for the visualization of protein by fluorescent microscopy.

0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)

CyはAmersham Biosciences Limited.からライセンスを受けて提供しています。

Cy3 is a trademark of Cytiva

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.