D9785
DMEM/F-12
With HEPES, without ʟ-glutamine, ʟ-leucine, ʟ-lysine, ʟ-methionine, CaCl2, MgCl₂, MgSO4, sodium bicarbonate, and phenol red, powde, suitable for cell cutlure
別名:
ダルベッコ改変イーグル培地/栄養混合物F-12ハム, DME/F-12, 1:1混合物
製品名
DMEM/栄養物ミクスチャ F-12 Ham, With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl2, MgCl2, MgSO4, sodium bicarbonate, and phenol red, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: no
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
NaHCO3: no
phenol red: no
glucose: 3.15 g/L
shipped in
ambient
storage temp.
2-8°C
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General description
もっとも基礎的な処方で提供されています。この処方は、(1;1)DME/ハムF-12の必須成分からスタートし、独自の用途に応じて処方を柔軟に最適化したいと考える研究者によって用いられています。.この基本処方は、標識されたL-アミノ酸を用いた細胞タンパク質代謝の研究に特に適しています。
Preparation Note
0.1545 g/L 塩化カルシウム、0.365 g/L L-グルタミン、0.05905 g/L L-ロイシン、0.09125 g/L L-リシン、0.0612 g/L 塩化マグネシウム、0.04884 g/L 硫酸マグネシウム、0.0172 g/L L-メチオニン、1.2 g/ L炭酸水素ナトリウムを添加してください。
14.8 gの粉末で1 Lの培地ができる配合です。
保管分類
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Vincent J Munster et al.
Nature, 585(7824), 268-272 (2020-05-13)
An outbreak of coronavirus disease 2019 (COVID-19), which is caused by a novel coronavirus (named SARS-CoV-2) and has a case fatality rate of approximately 2%, started in Wuhan (China) in December 20191,2. Following an unprecedented global spread3, the World Health Organization
Yuuma Furutani et al.
Cell biochemistry and function, 29(7), 577-581 (2011-08-23)
Magnesium (Mg) deficiency induces the production of free radicals, increases cytosolic ionized calcium concentration, and modulates the function of skeletal muscle in rats. The present study examined the effects of Mg deficiency on the gene expression of molecules related to
Huntingtin promotes mTORC1 signaling in the pathogenesis of Huntington's disease.
Pryor WM, Biagioli M, Shahani N, et al.
Science Signaling, 7(349), ra103-ra103 (2014)
Wei Ye et al.
Frontiers in microbiology, 11, 1105-1105 (2020-07-01)
Human enteroviruses are responsible for diverse diseases, from mild respiratory symptoms to fatal neurological complications. Currently, no registered antivirals have been approved for clinical therapy. Thus, a therapeutic agent for the enterovirus-related disease is urgently needed. Remdesivir (GS-5734) is a
Rui Yan et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 103, 1490-1497 (2018-06-06)
Neonatal brain hypoxia is a disease that affects the nervous system in children. Salidroside is a compound that has an anti-hypoxic effect, but the mechanism of salidroside in neonatal cerebral hypoxia is unclear. Hence, we investigated the regulatory effect and
資料
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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