E0639
エンドグリコシダーゼF2 from Elizabethkingia miricola
recombinant, expressed in E. coli, 20 U/mg
別名:
エリザベトキンギア・ミリコーラ, エンド-β-N-アセチルグルコサミニダーゼF2、エンドF2, エンドグリコシダーゼF2 Chryseobacterium meningosepticum由来, エンドグリコシダーゼF2 Elizabethkingia meningoseptica由来, エンドグリコシダーゼF2 Flavobacterium meningosepticum由来
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この商品について
CAS番号:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Specific activity:
20 U/mg
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Level
conjugate
(N-linked)
form
solution
specific activity
20 U/mg
mol wt
32 kDa
shipped in
wet ice
storage temp.
2-8°C
Packaging
5×反応バッファ-、250mM酢酸ナトリウム(pH 4.5)が付属しています。
Physical form
滅菌充填溶液 (10mM酢酸ナトリウム, 25mM塩化ナトリウム, pH 4.5)。
Other Notes
1 unitは、pH 4.5、37°C、1分間に、N-結合型オリゴ糖を1 μmolのブタ変性フィブリノーゲンから遊離させる酵素量です。
保管分類
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
資料
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
関連コンテンツ
Instructions
Liv Anette Bøhle et al.
FEMS microbiology letters, 325(2), 123-129 (2011-11-19)
It has been demonstrated previously that Enterococcus faecalis produces secreted endoglycosidases that enable the bacteria to remove N-linked glycans from glycoproteins. One enzyme potentially responsible for this activity is EF0114, comprising a typical GH18 endoglycosidase domain and a GH20 domain.
Helena Ryšlavá et al.
The FEBS journal, 278(14), 2469-2484 (2011-05-14)
Fungal β-N-acetylhexosaminidases are inducible extracellular enzymes with many biotechnological applications. The enzyme from Penicillium oxalicum has unique enzymatic properties despite its close evolutionary relationship with other fungal hexosaminidases. It has high GalNAcase activity, tolerates substrates with the modified N-acyl group
Shu-Quan Fan et al.
The Journal of biological chemistry, 287(14), 11272-11281 (2012-02-10)
Endo-β-N-acetylglucosaminidase from Streptococcus pneumoniae (Endo-D) is an endoglycosidase capable of hydrolyzing the Fc N-glycan of intact IgG antibodies after sequential removal of the sialic acid, galactose, and internal GlcNAc residues in the N-glycan. Endo-D also possesses transglycosylation activity with sugar