E2761
ExtrAvidin®−FITC
buffered aqueous solution
conjugate
FITC conjugate
form
buffered aqueous solution
extent of labeling
3-6 mol fluorochrome per mol protein
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20 μg/mL using human tissues, indirect immunofluorescence: 1:200
shipped in
dry ice
storage temp.
−20°C
Quality Level
関連するカテゴリー
General description
Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin is prepared from egg white avidin. It is a modified form of affinity purified avidin and combines high specific activity of avidin with the low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin has been conjugated to fluorescein isothiocyanate (FITC) and is used for various techniques.
Application
ExtrAvidin®-FITC is suitable for the following applications:
- immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a concentration of 20μg/mL using human tissues.
- Immunofluorescence†
- competitive binding assay
Physical form
0.01M PBS溶液 (10%(v/v)0.5M炭酸バッファ-, pH 9.5, 15mMアジ化ナトリウム含有)。
Preparation Note
アフィニティ-精製タンパク質。
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
E2761-.2ML: + E2761-1ML: + E2761-VAR: + E2761-BULK:
jan
Expression and localization of urokinase-type plasminogen activator receptor in bovine cumulus--oocyte complexes
Garcia DC
Zygote, 24, 230-235 (2016)
Maria Cristina D Picardo et al.
The Journal of physiology, 591(10), 2687-2703 (2013-03-06)
Breathing in mammals depends on an inspiratory-related rhythm that is generated by glutamatergic neurons in the pre-Bötzinger complex (preBötC) of the lower brainstem. A substantial subset of putative rhythm-generating preBötC neurons derive from a single genetic line that expresses the
D R Hampson et al.
The Journal of biological chemistry, 274(47), 33488-33495 (1999-11-24)
Metabotropic glutamate receptors (mGluRs) are G-protein-coupled glutamate receptors that subserve a number of diverse functions in the central nervous system. The large extracellular amino-terminal domains (ATDs) of mGluRs are homologous to the periplasmic binding proteins in bacteria. In this study
J L Tremoleda et al.
European journal of nuclear medicine and molecular imaging, 43(9), 1710-1722 (2016-05-08)
Traumatic spinal cord injury (SCI) is a devastating condition which affects millions of people worldwide causing major disability and substantial socioeconomic burden. There are currently no effective treatments. Modulating the neuroinflammatory (NI) response after SCI has evolved as a major
Xueying Wang et al.
eLife, 3, e03427-e03427 (2014-07-17)
To understand the neural origins of rhythmic behavior one must characterize the central pattern generator circuit and quantify the population size needed to sustain functionality. Breathing-related interneurons of the brainstem pre-Bötzinger complex (preBötC) that putatively comprise the core respiratory rhythm
関連コンテンツ
Instructions
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