F6162
Anti-Fibroblast Growth Factor-Basic antibody, Mouse monoclonal
clone FB-8, purified from hybridoma cell culture
別名:
Monoclonal Anti-Fibroblast Growth Factor-Basic antibody produced in mouse, Anti-FGF2
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この商品について
Conjugate:
unconjugated
Clone:
FB-8, monoclonal
Application:
DB, ELISA (i), Neutral, RIA, WB
Citations:
18
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
FB-8, monoclonal
form
buffered aqueous solution
mol wt
antigen 18 kDa
species reactivity
bovine, human
technique(s)
dot blot: suitable, indirect ELISA: suitable, neutralization: 5 μg/mL using 1 unit recombinant human FGF2 in mouse 3T3 cells, radioimmunoassay: suitable, western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FGF2(2247)
General description
Anti-Fibroblast Growth Factor-Basic antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the FB-8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Fibroblast growth factors (FGFs) are members of a family of polypeptides. The names FGF acidic and basic are used for FGF-1 and FGF-2, respectively. Acidic and basic fibroblast growth factor (aFGF and bFGF, respectively) are members of a small family of heparin binding growth factors (HBGF). bFGF usually has an apparent mass of about 18 kDa.
Immunogen
recombinant human FGF2.
Application
Anti-Fibroblast Growth Factor-Basic antibody has been used in in immunolabelling and western blotting. It may also be used in:
- in immunoblotting
- in enzyme linked immunosorbent assay (ELISA)
- in dot blot
- in radio immune assay (RIA)
- in neutralization bioassays
Used in protein pin array assay to identify the interactive sequences in human αB crystalline protein. The antibody can be used for neutralization of the biological activity of FGF2 in cell culture.
Biochem/physiol Actions
Fibroblast Growth Factor Basic (also known as bFGF) is a pleotropic cytokine that that is a potent mitogenic factor. On binding to its cognate receptor tyrosine kinase, FGFR, bFGF is capable of activating pathways such as MAPK and PI3K and produce cellular effects in varied cell types. Fibroblast Growth Factor-basic is reported to induce proliferation of fibroblasts, differentiation, wound healing and regeneration, limb formation, tissue remodeling and angiogenesis.
The antibody reacts specifically with both native and recombinant human FGF2. It does not cross-react with human FGF1.
Physical form
Solution, 0.2 μm filtered, in phosphate buffered saline, pH 7.4.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
F6162-VAR: + F6162-.2ML: + F6162-BULK:
jan
M Arras et al.
The Journal of clinical investigation, 101(1), 40-50 (1998-02-14)
We have previously shown that monocytes adhere to the vascular wall during collateral vessel growth (arteriogenesis) and capillary sprouting (angiogenesis). In this study we investigated the association of monocyte accumulation with both the production of the cytokines-basic fibroblast growth factor
Eric Sulpice et al.
Blood, 100(9), 3087-3094 (2002-10-18)
Platelet factor 4 (PF-4) is a member of the chemokine family with powerful antiangiogenic properties. The mechanism by which PF-4 inhibits endothelial cell proliferation is unclear. We investigated the effects of PF-4 on the intracellular signal transduction induced by basic
Ultrastructural immunolocalization of basic fibroblast growth factor in fibroblasts and extracellular matrix
Aktas RG and Kayton RJ
Histochemistry and Cell Biology, 113(3), 227-233 (2000)
An intrinsic quality-control mechanism ensures unconventional secretion of fibroblast growth factor 2 in a folded conformation
Torrado L C,et al.
Journal of Cell Science, 122(18), 3322-3329 (2009)
B P Mari et al.
The Journal of biological chemistry, 273(1), 618-626 (1998-02-07)
Stromelysin-3 (STR-3) is a recently characterized matrix metalloproteinase (MMP) with a unique pattern of expression and substrate specificity. Unlike other MMPs, STR-3 is consistently and dramatically overexpressed by multiple epithelial malignancies, including carcinomas of the breast, lung, colon, head and
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