F6636
栄養混合物 F-12ハム
Coon′s Modification, with ʟ-glutamine and zinc sulfate, without sodium bicarbonate, powder, suitable for cell culture
別名:
ハムF-12
製品名
栄養混合物 F-12ハム, powder, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bicarbonate, Coon′s Modification, suitable for cell culture
form
powder
technique(s)
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
components
phenol red: 0.00125 g/L
L-glutamine: 0.292 g/L
sodium pyruvate: 0.22 g/L
glucose: 1.802 g/L
shipped in
ambient
storage temp.
2-8°C
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Application
クーン改変ハムF-12は、ウイルス融合によって作製されたハイブリッド細胞を培養するために開発されました。この改変では、アミノ酸とピルビン酸が倍増され、アスコルビン酸が添加されています。また、塩濃度も変更されています。この処方は、硫酸亜鉛0.863 mg/Lを含んでいるため、マウスL-細胞の培養には不向きかもしれません。
Preparation Note
2.68 g/Lの重炭酸ナトリウムを添加してください。
培地1Lあたり粉末11.5 gが含まれる組成になっています。
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Feng Liang et al.
SLAS technology, 22(3), 315-324 (2017-04-06)
Cystic fibrosis (CF) is a hereditary disease caused by mutations in the gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR). A large number of nearly 2000 reported mutations, including the premature termination codon (PTC) mutations, urgently require new
Kenneth A Giuliano et al.
SLAS discovery : advancing life sciences R & D, 23(2), 111-121 (2017-09-13)
Cystic fibrosis (CF) is a lethal genetic disorder caused by mutation of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Despite recent groundbreaking approval of genotype-specific small-molecule drugs, a significant portion of CF patients still lack effective therapeutic options that
Xiaojiao Xue et al.
Human molecular genetics, 26(16), 3116-3129 (2017-06-03)
In-frame premature termination codons (PTCs) account for ∼11% of all disease-associated mutations. PTC suppression therapy utilizes small molecules that suppress translation termination at a PTC to restore synthesis of a full-length protein. PTC suppression is mediated by the base pairing
Wren E Michaels et al.
Nucleic acids research, 48(13), 7454-7467 (2020-06-11)
Cystic fibrosis (CF) is an autosomal recessive disorder caused by mutations in the CF transmembrane conductance regulator (CFTR) gene, encoding an anion channel that conducts chloride and bicarbonate across epithelial membranes. Mutations that disrupt pre-mRNA splicing occur in >15% of
Robert Rauscher et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(4) (2021-01-21)
Epistasis refers to the dependence of a mutation on other mutation(s) and the genetic context in general. In the context of human disorders, epistasis complicates the spectrum of disease symptoms and has been proposed as a major contributor to variations
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