F9175
Flt-3/Flk-2リガンド マウス由来
≥97% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
製品名
Flt-3/Flk-2リガンド マウス由来, ≥97% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
biological source
mouse
recombinant
expressed in NSO cells
assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
1.0-4.0 ng/mL
mol wt
~18 kDa
packaging
pkg of 5 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
mouse ... Flt3l(14256)
Analysis Note
生物活性は、Flt-3をトランスフェクションした前駆B細胞株の増殖に対する刺激能で測定しています。
糖鎖修飾が不均一であることから、リコンビナントマウスFlt-3リガンドは18-32 kDaの範囲に複数のバンドとして移動します。
General description
マウスとヒトのFlt-3リガンドは約72%のホモロジ-があり、ともに異種間で活性を発揮します。
Flt3l (FMS-like tyrosine kinase 3 ligand) is a cytokine and a hematopoietic growth factor. It acts as a ligand for FLT3 receptor. Alternative splicing of FLT3L results in both membrane-bound and soluble forms. Soluble form is a result of proteolytic cleavage of the membrane isoform. It has a wide range of tissue expression.
Physical form
凍結乾燥品(0.2μmフィルターで濾過滅菌済み、50%アセトニトリル、0.1%トリフルオロ酢酸溶液。サイトカイン1 μgにつき50 μgのウシ血清アルブミンを含有)。
Biochem/physiol Actions
Flt3l (FMS-like tyrosine kinase 3 ligand) acts as a stimulant of hematopoietic progenitor cell proliferation. It facilitates the commitment of lymphoid lineage, and aids in dendritic cell (DC) development. It is involved in myeloblast differentiation, and drives skeletal muscle regeneration. It acts on p120RasGAP and suppresses Erk signaling, which in turn promotes myogenesis. In lymphoid tissues, it modulates DC homeostasis, which is facilitated by steady state as well as proliferative T-cells.
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
F9175-20KG: + F9175-BULK: + F9175-5UG: + F9175-5UG-PW: + F9175-VAR:
jan
S D Lyman et al.
Current opinion in hematology, 2(3), 177-181 (1995-05-01)
The flt3 ligand is a member of a small family of growth factors that stimulate the proliferation of hematopoietic cells. Other members of this family include Steel factor (also known as mast cell growth factor, stem cell factor, and kit
Yasuyuki Saito et al.
European journal of immunology, 43(6), 1651-1658 (2013-03-23)
Lymphoid-tissue dendritic cells (DCs) are short-lived and need to be continuously replenished from bone marrow-derived DC progenitor cells. Fms-related tyrosine kinase 3 is expressed during cellular development from hematopoietic progenitors to lymphoid-tissue DCs. Fms-related tyrosine kinase 3 ligand (Flt3L) is
Yejing Ge et al.
Journal of cell science, 126(Pt 15), 3370-3379 (2013-05-25)
Various cues initiate multiple signaling pathways to regulate the highly coordinated process of skeletal myogenesis. Myoblast differentiation comprises a series of ordered events starting with cell cycle withdrawal and ending with myocyte fusion, with each step probably controlled by multiple
資料
Read article on hematopoietic cytokines and hematopoiesis
関連コンテンツ
Instructions
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