G0535
グリコペプチダーゼA from almonds
buffered aqueous glycerol solution, ≥0.05 unit/mL
別名:
N-グリコシダーゼA, N結合型-グリコペプチド-(N-アセチル-β-D-グルコサミニル)-L-アスパラギンアミドヒドロラーゼ, PNGアーゼ A
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この商品について
CAS番号:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Concentration:
≥0.05 unit/mL
conjugate
(N-linked)
form
buffered aqueous glycerol solution
concentration
≥0.05 unit/mL
storage temp.
−20°C
Quality Level
関連するカテゴリー
General description
Glycopeptidase found in almonds can be divided into three groups- A, B and C. the optimum pH value and the isoelectric point of glycopeptidase A is 6.0 and 7.7 respectively. It has a preference for glycopeptides with long chains. It is also capable of hydrolyzing intact glycoproteins such as, desialyted human transferrin, ovalbumin etc. These proteins cleave glycoproteins with asialocarbohydrate moieties at their β-aspartyl-glucosylamine linkages.
Application
Glycopeptidase A from almonds is used for deglycosylation. It catalyzes the removal of N-linked oligosaccharide chains and converts Asn residue to Asp.
Biochem/physiol Actions
N4-(アセチル-β-D-グリコサミニル)アスパラギンや、そのN-アセチル-D-グルコサミン残基がさらにグリコシル化されている化合物を加水分解し、(置換型)N-アセチル-β-D-グルコアミニルアミンとアスパラギン酸残基含有ペプチドを産生します。
Physical form
50 mMクエン酸-リン酸バッファー溶液(pH 5.0、50%グリセロール、 BSA含有)。
Other Notes
1 unitは、pH 5.0、37°C、1分間に、1.0μmolのオブアルブミン糖ペプチドを加水分解する酵素量です。
保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
G0535-.005UN-PW: + G0535-VAR: + G0535-.005UN: + G0535-BULK:
jan
T Takahashi et al.
Biochimica et biophysica acta, 657(2), 457-467 (1981-02-13)
The glycopeptidase preparation that has been isolated from almond emulsin and acts on beta-aspartylglycosylamine linkages in glycopeptides was separated into three active fractions by DEAE-cellulose column chromatography. The three discrete species of glycopeptidase (Groups A, B and C) have been
Asparagine-linked oligosaccharides in human placenta and umbilical cord as demonstrated by almond glycopeptidase.
N Takahashi et al.
FEBS letters, 146(1), 139-142 (1982-09-06)
R P Miller et al.
Biochimica et biophysica acta, 954(1), 50-57 (1988-04-28)
The beta-subunit of dog kidney (Na+ + K+)-ATPase is a sialoglycoprotein and contains three potential N-glycosylation sites. In this study, the oligosaccharide chains of purified dog kidney beta-subunit were labeled with tritium by oxidation with sodium periodate or galactose oxidase
Karen G Welinder et al.
The Journal of biological chemistry, 284(15), 9764-9769 (2009-02-13)
Proteome data of potato (Solanum tuberosum) tuber juice and of purified potato tuber vacuoles indicated that mature patatins may perhaps lack a C-terminal propeptide. We have confirmed this by complete mass spectrometric sequencing of a number of patatin variants as
Anna C Need et al.
Journal of medical genetics, 49(6), 353-361 (2012-05-15)
There is considerable interest in the use of next-generation sequencing to help diagnose unidentified genetic conditions, but it is difficult to predict the success rate in a clinical setting that includes patients with a broad range of phenotypic presentations. The
資料
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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