抗β-グルクロニダーゼ (C-末端) ウサギ宿主抗体

The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

本抗体は遺伝子導入タバコ植物で発現した細菌のGUSを認識します。

β-Glucuronidase (GUS) acts as a reporter gene for plant studies. Reporter genes are widely used for studying the expression of foreign genes in transformed plant tissues. GUS is an hydrolase that catalyzes the cleavage of a variety of β-glucuronide derivatives available for colorimetric, fluorometric and histochemical assays. GUS activity is easily assayed in vitro and can withstand fixation, enabling histochemical localization in cells and tissue sections. However, one of the major limitations of the gus reporter gene system is that the histochemical GUS assay system is destructive for the plant tissue, and therefore it is not suitable for direct visual selection of transformed plants.

Anit-β-Glucuronidase (C-Terminal) recognizes bacterial GUS expressed in transgenic tobacco plants.

E. coli GUSのC末端側アミノ酸589-603に相当する合成ペプチドのKLHに結合体。

0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)

Detection of GUS by immunoblotting (60 kDa). Staining of the GUS band in immunoblotting is specifically inhibited by the immunizing GUS peptide (E. coli, amino acids 589-603).

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β-Glucuronidase (GUS) gene (also referred to as uidA) from Escherichia- coli, codes for a 60kDa protein.