G6160
モノクロナール抗β-COP マウス宿主抗体
clone maD, ascites fluid
別名:
Anti-BARMACS, Anti-COPB
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この商品について
Conjugate:
unconjugated
Clone:
maD, monoclonal
Application:
ARR, IF, WB
Species reactivity:
human, rat, hamster, monkey
Citations:
25
Technique(s):
indirect immunofluorescence: 1:80 using cultured Chinese hamster ovary (CHO) cells, microarray: suitable, western blot: 1:1,000 using a preparation of stacked Golgi membranes from rat liver
Uniprot accession no.:
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
maD, monoclonal
contains
15 mM sodium azide
species reactivity
human, rat, hamster, monkey
technique(s)
indirect immunofluorescence: 1:80 using cultured Chinese hamster ovary (CHO) cells, microarray: suitable, western blot: 1:1,000 using a preparation of stacked Golgi membranes from rat liver
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... COPB1(1315)
rat ... Copb1(114023)
関連するカテゴリー
Biochem/physiol Actions
抗体はβ-COPタンパク質(110 kDa)のエピト-プを認識し、免疫細胞化学ではゴルジ複合体の周辺を染色します。
COPs (coatomer proteins) contain adaptin-like, complex (8) and are transiently attached to the vesicles involved in transport within the Golgi complex and possibly between the rough ER and Golgi complex. β-COP has a molar mass of 110kDa and its primary structure is homologous to the β-adaptin component of clathrin-coated vesicles.
Immunogen
合成β-COPペプチドD1(アミノ酸701-715)のKLH結合体。
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Monoclonal Anti-β-COP antibody produced in mouse is suitable for use as a primary antibody in immunoblot:
It is suitable for immunostaining of β-coatomer, that is used as a intracellular, Golgi protein marker :
It is suitable for use in cell-surface ELISA of human aortic endothelial cells (HAEC)
It is also suitable for western blot analysis at a working dilution of 1:1000 using a preparation of stacked Golgi membranes from rat liver, for indirect immunofluorescence at a working dilution of 1:80 using cultured Chinese hamster ovary (CHO) cells and for microarray.
- analysis at a working dilution of 1:1000 using subcellular proteins from rat PC12 (pheochromocytoma) cells
- analysis of gradient fractions of cerebral microvessels to confirm the separation of plasma membrane lipid raft domains from intracellular membranous components
- detection of the Golgi marker protein β-COP in exosome-enriched extracellular microvesicles (eMV) preparations from untreated HeLa cells
It is suitable for immunostaining of β-coatomer, that is used as a intracellular, Golgi protein marker :
- to confirm that CD14 staining is localized to the cell surface of HAEC
- for examining the localization of Meltrin β in the Golgi apparatus and its vicinity in neurons prepared from developing dorsal root ganglia of mouse embryos
- in NB4 and NB4-LR1 cells to examine the colocalization of PKA regulatory subunits
- in CHO cells to examine colocalization of GFP-Rab24
It is suitable for use in cell-surface ELISA of human aortic endothelial cells (HAEC)
It is also suitable for western blot analysis at a working dilution of 1:1000 using a preparation of stacked Golgi membranes from rat liver, for indirect immunofluorescence at a working dilution of 1:80 using cultured Chinese hamster ovary (CHO) cells and for microarray.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
The coatomer (approx. 550kDa) consists of proteins designated α-, β-, γ-, and δ-COP, together with substoichiometric amounts of several other proteins.
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保管分類
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
G6160-100UL: + G6160-VAR: + G6160-.5ML: + G6160-.2ML: + G6160-BULK:
jan
Fumi Kano et al.
Journal of cell science, 122(Pt 13), 2218-2227 (2009-06-11)
Yip1A, a mammalian homologue of yeast Yip1p, is a multi-spanning membrane protein that is considered to be involved in transport between the endoplasmic reticulum (ER) and the Golgi. However, the precise role of Yip1A in mammalian cells remains unclear. We
J W Creemers et al.
The Journal of biological chemistry, 276(6), 4211-4217 (2000-11-10)
The amyloid peptide is the main constituent of the amyloid plaques in brain of Alzheimer's disease patients. This peptide is generated from the amyloid precursor protein by two consecutive cleavages. Cleavage at the N terminus is performed by the recently
Daniela B Munafó et al.
Traffic (Copenhagen, Denmark), 3(7), 472-482 (2002-06-06)
Rab GTPases comprises a large family of proteins, with more than 50 gene products localized in distinct subcellular compartments. Rab24 is a member of this family whose function is not presently known. In order to elucidate the role of this
Gwen McCaffrey et al.
Journal of neurochemistry, 106(6), 2395-2409 (2008-07-24)
Tight junctions (TJs) at the blood-brain barrier (BBB) dynamically alter paracellular diffusion of blood-borne substances from the peripheral circulation to the CNS in response to external stressors, such as pain, inflammation, and hypoxia. In this study, we investigated the effect
Kimberly A Walton et al.
The Journal of biological chemistry, 278(32), 29661-29666 (2003-06-05)
We demonstrated previously that oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (ox-PAPC) and, specifically, the component lipid 1-palmitoyl-2-(5,6-epoxyisoprostane E2)-sn-glycero-3-phosphorylcholine increase interleukin-8 (IL-8) synthesis in aortic endothelial cells. The goal of the current studies was to characterize the receptor complex mediating the increased transcription of IL-8.
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