製品名
PerfectHyb™ Plusハイブリダイゼーションバッファー, for Northern and Southern blotting, solution
form
solution
storage temp.
room temp
Quality Level
Application
ノーザンブロット、サザンブロット、コロニー/プラークブロット、およびドットブロットに用いるハイブリダイゼーション緩衝液への使用に適しています。
Features and Benefits
- 迅速で使いやすい
- 従来の緩衝液に比べて最大20倍高感度
- 遮断ステップが不要
- 中性およびプラスに帯電した膜に適合
- 化学発光、比色、およびDIG検出アッセイに適合
- 放射性および非放射性プローブに適合
- 一本鎖または二本鎖DNA、RNA、およびオリゴヌクレオチドのプローブに適合
General description
シグマのPerfectHyb Plusは、ノーザンブロットおよびサザンブロットプロトコール用の調製済みハイブリダイゼーション緩衝液です。 PerfectHyb Plusを使用すると、ハイブリダイゼーション時間をわずか2時間に短縮可能です。
Other Notes
溶液中に生じた少量の沈殿は、30~35°Cで時々静かに転倒撹拌しながら溶解させることができます。
Legal Information
PerfectHyb is a trademark of Sigma-Aldrich Co. LLC
保管分類
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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RNA (New York, N.Y.), 15(7), 1258-1264 (2009-05-23)
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Angel Emilio Martínez de Alba et al.
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In plants, most microRNAs (miRNAs) and several endogenous small interfering RNAs (siRNAs) bind to ARGONAUTE1 (AGO1) to regulate the expression of endogenous genes through post-transcriptional gene silencing (PTGS). AGO1 also participates in a siRNA-mediated PTGS defense response that thwarts exogenous
プロトコル
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
関連コンテンツ
Instructions
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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