H9286

抗アセチル-ヒストンH3 (Ac-Lys⁹) ウサギ宿主抗体

Name: 抗アセチル-ヒストンH3 (Ac-Lys<SUP>9</SUP>) ウサギ宿主抗体
Brand: SIGMA

2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

別名:

Anti-H3K9ac

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この商品について

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02685921

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IF, WB

Citations:

主要文書

すべての文書を表示

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テクニカルサービス

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~17 kDa

species reactivity

bovine, human, Caenorhabditis elegans, frog, mouse, rat, Drosophila, chicken

concentration

2-2.5 mg/mL

technique(s)

indirect immunofluorescence: 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate, microarray: suitable, western blot: 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate

UniProt accession no.

P68431

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

acetylation (Lys9)

Quality Level

200

General description

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit is suitable for:

indirect immunofluorescence at a dilution of 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate
microarray
western blot at a dilution of 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate

Immunogen

ヒトヒストンH3のN末端に相当する合成[Ac-Lys⁹]アセチル化ヒストンH3ペプチド(アミノ酸7-20)。この配列はマウス, ラット, ウシ, ニワトリ, カエル, Drosophila, C. elegansなど多くの生物種で同一であり、TetrahymenaヒストンH3でも高度に保存されている(1アミノ酸置換)。

Application

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit has been used in:

indirect immunofluorescence
immunoblotting
immunohistochemistry

Biochem/physiol Actions

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers.

In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23. Acetylation at lysine 9 significantly influences histone deposition and chromatin assembly in some organisms. Acetylation of specific lysine residues in H3 is also associated with processes apart from transcription.

Physical form

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

H9286-200UL: + IXO11355: + H9286-VAR: + H9286-BULK:

jan

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number

0000510782

0000462431

0000232746

適切なバージョンが見つかりませんか。

特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

COAを検索

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

p66shc negatively regulates insulin-like growth factor I signal transduction via inhibition of p52shc binding to Src homology 2 domain-containing protein tyrosine phosphatase substrate-1 leading to impaired growth factor receptor-bound protein-2 membrane recruitment.

Gang Xi et al.

Molecular endocrinology (Baltimore, Md.), 22(9), 2162-2175 (2008-07-09)

Our previous studies have indicated an essential role of p52shc in mediating IGF-I activation of MAPK in smooth muscle cells (SMC). However, the role of the p66 isoform of shc in IGF-I signal transduction is unclear. In the current study

Social isolation-induced epigenetic changes in midbrain of adult mice

Siuda D, et al.

Journal of Physiology And Pharmacology, 65(2), 247-255 (2014)

The role of Src kinase in insulin-like growth factor-dependent mitogenic signaling in vascular smooth muscle cells.

Jaroslava Lieskovska et al.

The Journal of biological chemistry, 281(35), 25041-25053 (2006-07-11)

Activation of the MAPK pathway mediates insulin-like growth factor-I (IGF-I)-dependent proliferation in vascular smooth muscle cells (SMC). Our previous studies have shown that IGF-I-induced Shc phosphorylation is necessary for sustained activation of MAPK and increased cell proliferation of SMCs, and

Histone H4 acetylation is essential to proceed from a histone- to a protamine-based chromatin structure in spermatid nuclei of Drosophila melanogaster.

Stephan Awe et al.

Systems biology in reproductive medicine, 56(1), 44-61 (2010-02-23)

In humans, other mammals, and also in Drosophila, the paternal genome in the sperm is highly condensed and organized mainly in a protamine-based chromatin structure. However, the timing and mechanism of the switch from a histone- to the protamine-based chromatin

The language of covalent histone modifications

Strahl BD and Allis CD

Nature, 403(6765), 41-41 (2000)

抗アセチル-ヒストンH3 (Ac-Lys9) ウサギ宿主抗体

2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

サイズを選択してください

この商品について

主要文書

特性

biological source

conjugate

antibody form

antibody product type

clone

form

mol wt

species reactivity

concentration

technique(s)

UniProt accession no.

shipped in

storage temp.

target post-translational modification

Quality Level

Gene Information

関連するカテゴリー

詳細

General description

Immunogen

Application

Biochem/physiol Actions

Physical form

Disclaimer

安全性情報

保管分類

wgk

flash_point_f

flash_point_c

適用法令

H9286-200UL: + IXO11355: + H9286-VAR: + H9286-BULK:

資料

試験成績書（COA）

以前この製品を購入いただいたことがある場合

参考文献

プロトコルと関連資料

関連コンテンツ

テクニカルサービス

抗アセチル-ヒストンH3 (Ac-Lys⁹) ウサギ宿主抗体