トランスフォーミング増殖因子-β1潜在型結合ペプチド ヒト

Research Area: Cell Signaling

TGF-β is released from degranulating platelets and secreted from nearly all cells in a biologically inactive complex which is unable to bind to cellular receptors and is not recognized by antibodies to TGF-β. The peptide can be activated by acidification, alkalinization, or action of chaotropic agents in vitro. The complex consists of TGF-β associated non-covalently with a protein designated as the latency associated peptide (LAP). TGF-β and LAP represent components of a pro-peptide that is cleaved in a post-golgi compartment prior to secretion. The recombinant human LAP is produced from a DNA sequence corresponding to the 278 amino acid residues of pre-pro-TGF-β1 terminating prior to the mature TGF-β1. LAP contains a Cys33 to Ser33 substitution. LAP contains 249 amino acids, generated after cleavage of a 29 amino acid residue signal peptide. LAP is a glycoslyated, disulfide linked homodimer.

Latency Associated Peptide (LAP) may be used to inhibit transforming growth factor-β 1 activity in various biological systems.

Transforming Growth Factor-β1 Latency Associated Peptide human has been used to coat the cell culture inserts for cell migration assays to determine the functional effects of impeding αvβ6 endocytosis in vitro. It has also been used to coat non-tissue culture-treated 96-well plates for adhesion assays.

TGF-β1は多種の細胞から産生されますが、哺乳動物の血小板における濃度が最も高いと報告されています。

TGF-β1は多種の細胞から産生されますが、哺乳動物の血小板における濃度が最も高いと報告されています。哺乳動物の血小板には、TGF-β2の約4倍のTGF-β1が存在します。

Latency Associated Peptide (LAP) binds transforming growth factor-beta 1 (TGF-β1) to form a latent complex. The activity of TGF-β1 is primarily regulated through the activation of the latent molecule. LAP has the ability to stimulate epithelial cell migration, representing a new function for LAP in controlling monocyte trafficking and immune modulation. Following post-translational modification, TGF-β1 binds non-covalently to the LAP to establish latency. Due to its crucial function in modulating TGF-β1 activity, LAP plays a fundamental role in governing a range of TGF-β1 effects. LAP is expressed on immature dendritic cells and contributes to T cell differentiation. LAP exhibits both chemotactic and anti-inflammatory activity independently of active TGF-β1.

凍結乾燥品(PBS溶液, 1.25mg BSA含有, 0.2μmフィルタ-濾過済み)。

生物活性は、マウスHT-2細胞のTGF-β1阻害能により測定しています。

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