M0275
イーグル最少必須培地
With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x
別名:
MEM
製品名
イーグル最少必須培地, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
concentration
10 ×
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
Quality Level
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Application
FBS、子ウシまたはウマ血清の存在下で、線維芽細胞などの付着細胞株を増殖させるために一般的に使用されます。L-グルタミンおよび炭酸水素ナトリウムを1倍に希釈した後培地に添加し、pHを調整します。
イーグル最少必須培地はコラーゲンメタクリルアミド（ヒドロゲル）の製剤に使用されています。また、後に MRI（磁気共鳴映像法）研究や組織学的分析に用いられるウシ膝の膝関節軟骨の維持にも用いられています。
Preparation Note
1倍培地に対して0.292 g/LのL-グルタミンおよび2.2 g/Lの重炭酸ナトリウムを添加してください。
保管分類
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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資料
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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