M6818
Anti-MeCP2 antibody, Mouse monoclonal
clone Mec-168, purified from hybridoma cell culture
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この商品について
Conjugate:
unconjugated
Clone:
Mec-168, monoclonal
Application:
immunocytochemistry
indirect ELISA
microarray
western blot
indirect ELISA
microarray
western blot
Species reactivity:
rat, human, mouse
Citations:
26
Technique(s):
immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma.
indirect ELISA: suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma.
Uniprot accession no.:
製品名
Anti-MeCP2 antibody, Mouse monoclonal, clone Mec-168, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
Mec-168, monoclonal
form
buffered aqueous solution
mol wt
antigen ~75 kDa
species reactivity
rat, human, mouse
packaging
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MECP2(4204)
mouse ... Mecp2(17257)
rat ... Mecp2(29386)
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Western Blotting (1 paper)
Immunoprecipitation (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-MeCP2 antibody produced in mouse has been used in:
- immunoblotting
- immunoprecipitation
- immunostaining
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
- western blot (0.1-0.2 μg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma)
Biochem/physiol Actions
Methyl-CpG binding protein 2 (MeCP2) is a transcription modulator that binds methylated DNA. This protein regulates neuronal functions and central nervous system development. Alterations in MeCP2 have been associated with neurological diseases such as MECP2 duplication syndrome and Rett syndrome
Methyl-CpG binding protein 2 (MeCP2) deacetylase (HDAC) repressive machinery via recruitment of the Sin 3A corepressor, thus removing acetyl groups from histones and consequently, silencing genes. Antibodies reacting specifically with MeCP2 may be used for studying chromatin remodeling effects on gene expression.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-MeCP2 (mouse IgG1 isotype) is derived from the Mec-168 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C- terminus of human MeCP2. Methyl-CpG binding protein 2 (MeCP2) is the first methyl-CpG-binding protein to be isolated. This protein contains a methyl-CpG-binding domain (MBD) and a transcriptional repression domain (TRD).
Immunogen
synthetic peptide corresponding to the C-terminus (amino acids 471-486) of human MeCP2.
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保管分類
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
M6818-BULK: + M6818-100UL: + M6818-VAR: + M6818-200UL: + M6818-25UL:
jan
The molecular basis of variable phenotypic severity among common missense mutations causing Rett syndrome
Brown K, et al.
Human Molecular Genetics, 25(3), 558-570 (2015)
Xiangling Meng et al.
eLife, 5 (2016-06-22)
Many postnatal onset neurological disorders such as autism spectrum disorders (ASDs) and intellectual disability are thought to arise largely from disruption of excitatory/inhibitory homeostasis. Although mouse models of Rett syndrome (RTT), a postnatal neurological disorder caused by loss-of-function mutations in
Rett syndrome mutations abolish the interaction of MeCP2 with the NCoR/SMRT co-repressor
Lyst MJ, et al.
Nature Neuroscience, 16(7) (2013)
The biological functions of the methyl-CpG-binding protein MeCP2 and its implication in Rett syndrome
Nan X, et al.
Brain & Development, 23, S32-S37 (2001)
Oz Pomp et al.
Cell stem cell, 9(2), 156-165 (2011-08-06)
Somatic tissues in female eutherian mammals are mosaic due to random X inactivation. In contrast to mice, X chromosome reactivation does not occur during the reprogramming of human female somatic cells to induced pluripotent stem cells (iPSCs), although this view
関連コンテンツ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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