M7278
イーグル最少必須培地
HEPES modification, with Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
別名:
MEM
製品名
イーグル最少必須培地, HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
>7.2
components
L-glutamine: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: yes
shipped in
ambient
storage temp.
2-8°C
Quality Level
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Application
イーグル最小必須培地は、原始卵胞や卵巣組織、ヒト皮膚線維芽細胞、ヒト結腸上皮細胞株LS174T、および海馬組織切片の培養に使用されています。
General description
イーグル最小必須培地は、糖、無機塩、水溶性ビタミン、および必須アミノ酸で構成されています。多くの細胞培養研究の培地処方として広く使用されています。
Preparation Note
0.292 g/LのL-グルタミンを添加してください。
保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Shafagh A Waters et al.
The EMBO journal, 36(3), 374-387 (2016-11-12)
RNA sequencing studies have identified hundreds of non-coding RNAs in bacteria, including regulatory small RNA (sRNA). However, our understanding of sRNA function has lagged behind their identification due to a lack of tools for the high-throughput analysis of RNA-RNA interactions
Samuel Wilson Failor et al.
Neural development, 13(1), 4-4 (2018-03-27)
Activity in neurons drives afferent competition that is critical for the refinement of nascent neural circuits. In ferrets, when an eye is lost in early development, surviving retinogeniculate afferents from the spared eye spread across the thalamus in a manner
Rodrigo Lerchundi et al.
Frontiers in cellular neuroscience, 14, 80-80 (2020-05-07)
Genetically encoded nanosensors such as the FRET-based adenosine triphosphate (ATP) sensor ATeam enable the measurement of changes in ATP levels inside cells, promoting our understanding of metabolic interactions between astrocytes and neurons. The sensors are usually well characterized in vitro
Alfred K Yu et al.
Human molecular genetics, 24(10), 2848-2860 (2015-02-06)
Mitochondrial complex I (NADH dehydrogenase) is a major contributor to neuronal energetics, and mutations in complex I lead to vision loss. Functional, neuroanatomical and transcriptional consequences of complex I deficiency were investigated in retinas of the Ndufs4 knockout mouse. Whole-eye
Tatsuma Yao et al.
Reproductive medicine and biology, 16(2), 99-117 (2017-12-21)
Cell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal-cell culture media. A literature search was performed on PubMed and Google
資料
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
関連コンテンツ
Instructions
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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