N1038
モノクロナール抗Nup98
clone 2H10, purified from hybridoma cell culture
別名:
Anti-Nucleoporin, 98-kD
ログインで組織・契約価格をご覧ください。
サイズを選択してください
この商品について
Conjugate:
unconjugated
Clone:
2H10, monoclonal
Application:
immunocytochemistry
indirect ELISA
western blot
indirect ELISA
western blot
Species reactivity:
rat, human
Citations:
11
Technique(s):
immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using HeLa nuclear extract
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using HeLa nuclear extract
Uniprot accession no.:
製品名
モノクロナール抗Nup98, clone 2H10, purified from hybridoma cell culture
biological source
rat
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2H10, monoclonal
form
buffered aqueous solution
mol wt
antigen ~98 kDa
species reactivity
rat, human
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using HeLa nuclear extract
isotype
IgG2cκ
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NUP98(4928)
rat ... Nup98(81738)
Application
Anti-Nup98 antibody, Rat monoclonal may be used in:
- enzyme linked immunosorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
Biochem/physiol Actions
Nucleoporin-98kDa (Nup98) is involved in the translocation of RNA and proteins across the nucleus. It is also a regulator of chromosomal segregation and gene expression. The protein also binds to other nucleoporins and is associated with acute myelogenous leukemia.
Nup98 functions as a docking protein for cytosol mediated docking of import substrates. Securin protein damage caused by the anaphase-promoting complex (APC) during mitosis, is regulated by the nucleocytoplasmic transport factors Rae1 and Nup98. In mice, collective haploinsufficiency of Rae1 and Nup98 gene causes premature separation of sister chromatids and severe aneuploidy. In addition, it also leads to untimely degradation of securin. Chromosome rearrangement between the HOXA9 (homeobox A9) gene and the nucleoporin gene Nup98 has been observed in some human myeloid leukemia patients.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Nup98 (rat IgG2c- isotype) is derived from the hybridoma 2H10 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from WKY/NCrj rat immunized with a recombinant fragment of human Nup98. Nup96 and Nup98 are the nucleoporins that are formed by the cleavage of 186 kDa precursor protein.
Nucleoporin-98kDa (Nup98) is part of the nuclear pore complex. It possesses two domains and the gene encoding it is localized on human chromosome 11.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
N1038-200UL: + N1038-25UL: + N1038-100UL: + N1038-VAR: + N1038-BULK:
jan
Céline Ricour et al.
Journal of virology, 83(21), 11223-11232 (2009-08-28)
The leader protein of cardioviruses, Theiler's murine encephalomyelitis virus (TMEV) and encephalomyocarditis virus (EMCV), is a multifunctional protein known to antagonize type I interferon expression and to interfere with nucleocytoplasmic trafficking of host proteins and mRNA. This protein plays an
Takaomi Fukuhara et al.
Hybridoma (2005), 24(5), 244-247 (2005-10-18)
Nup98 is a component of nuclear pore complexes, which are large protein assemblies embedded in the nuclear envelope. Previous studies have shown that Nup98 interacts with several transport factors and plays a critical part in nuclear trafficking. However, the mechanism
Masaaki Iwamoto et al.
Genes to cells : devoted to molecular & cellular mechanisms, 15(7), 661-669 (2010-06-16)
The nucleoporin Nup98 is an essential component of the nuclear pore complex. This peripheral nucleoporin with its Gly-Leu-Phe-Gly (GLFG) repeat domain contributes to nuclear-cytoplasmic trafficking, including mRNA export. In addition, accumulating studies indicate that Nup98 plays roles in several important
NUP98 gene fusions and hematopoietic malignancies: common themes and new biologic insights
Gough SM, et al.
Blood, 118(24), 6247-6257 (2011)
Valentina Salsi et al.
Cancer research, 74(4), 1079-1090 (2013-12-29)
NUP98 is a recurrent fusion partner in chromosome translocations that cause acute myelogenous leukemia. NUP98, a nucleoporin, and its interaction partner Rae1, have been implicated in the control of chromosome segregation, but their mechanistic contributions to tumorigenesis have been unclear.
関連コンテンツ
Instructions
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）