N5386
ミクロコッカスヌクレアーゼ Staphylococcus aureus由来
100-300 units/mg protein
別名:
MNase, エンドヌクレアーゼ, ミクロコッカス由来, ミクロコッカスエンドヌクレアーゼ
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この商品について
CAS番号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-748-0
MDL number:
Specific activity:
100-300 units/mg protein
Biological source:
Staphylococcus aureus
biological source
Staphylococcus aureus
form
powder
specific activity
100-300 units/mg protein
composition
Protein, ≥40% E1%/280 (, Balance primarily sodium acetate)
technique(s)
DNA extraction: suitable, DNA purification: suitable
suitability
suitable for molecular biology
application(s)
cell analysis
storage temp.
−20°C
Quality Level
Gene Information
Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)
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Application
Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes.
Nuclease micrococcal from Staphylococcus aureus has been used in a study to monitor hybridization reactions with DNA. It has also been used in a study to investigate the optimal conditions for assay of staphylococcal nuclease.
Biochem/physiol Actions
DNAの5′-ホスホジエステル結合を加水分解します。
Other Notes
注:1 μmolユニット = 約85 A260ユニットです。ここで、1 A260ユニットは、反応液量が3.55 mLの場合に、pH 8.8、37°C、30分間に、ΔA260が1.0となる酵素量です。光路長 = 1 cm。
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
N5386-BULK: + N5386-50UN: + N5386-50UN-PW: + N5386-200UN: + N5386-VAR: + N5386-200UN-PW: + N5386-500UN: + N5386-500UN-PW:
jan
Optimal conditions for assay of staphylococcal nuclease
KAMMAN, J. and S. TATINI
Journal of Food Science, 42, 421-424 (1977)
Ho-Ryun Chung et al.
PloS one, 5(12), e15754-e15754 (2011-01-06)
Eukaryotic genomes are packed into chromatin, whose basic repeating unit is the nucleosome. Nucleosome positioning is a widely researched area. A common experimental procedure to determine nucleosome positions involves the use of micrococcal nuclease (MNase). Here, we show that the
Ashish Kumar Singh et al.
Nature communications, 12(1), 7011-7011 (2021-12-03)
Numerous chromatin remodeling enzymes position nucleosomes in eukaryotic cells. Aside from these factors, transcription, DNA sequence, and statistical positioning of nucleosomes also shape the nucleosome landscape. The precise contributions of these processes remain unclear due to their functional redundancy in
Frank J Hernandez et al.
Nature medicine, 20(3), 301-306 (2014-02-04)
Technologies that enable the rapid detection and localization of bacterial infections in living animals could address an unmet need for infectious disease diagnostics. We describe a molecular imaging approach for the specific, noninvasive detection of S. aureus based on the
Gang Wei et al.
Methods in enzymology, 513, 297-313 (2012-08-30)
Gene transcription can be regulated through alteration of chromatin structure, such as changes in nucleosome positioning and histone-modification patterns. Recent development of techniques based on the next-generation sequencing technology has allowed high-resolution analysis of genome-wide distribution of these chromatin features.
関連コンテンツ
QC Methods
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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