モノクロナール抗p27^Kip1 マウス宿主抗体

Monoclonal Anti p27Kip1 (mouse IgG1 isotype) is derived from the DCS-72 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant p27Kip1 protein of rodent origin.

齧歯類のリコンビナントp27^Kip1タンパク質。

Monoclonal Anti-p27Kip1 antibody produced in mouse has been used in immunoblotting, immunofluorescence, immunohistochemistry, immunoprecipitation and immunocytochemistry.

イムノブロッティングの場合、サンプルによっては高分子量および低分子量の分子のバンドがみられる可能性もあります。これらは、p27^Kip1がポリユビキチン化されているか、あるいは切断産物であると考えられます。

p27, the product of the kinase inhibition protein1 (kip1) gene, is an inducible inhibitor of cyclin dependent kinase activity. p27Kip1 interacts strongly with D-type cyclins complexed with CDK4 and more weakly with cyclin E and CDK2 complexes. Regulation by p27Kip1 may be an essential step in the pathway that links mitogenic signals to cell cycle progression and may be a key molecular event in the physiological process of cell cycle commitment or passage through the restriction point. Thus, the amount of p27Kip1 increases in quiescent cells and rapidly decreases after stimulation with specific mitogens. Constitutive expression of p27Kip1 in cultured cells causes cell cycle arrest in G1.

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