P2860
モノクロナール抗PSF マウス宿主抗体
clone B92, purified from hybridoma cell culture
別名:
Mouse Anti-Polypyrimidine tract-binding protein-associated splicing factor
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この商品について
Conjugate:
unconjugated
Clone:
B92, monoclonal
Application:
ARR, ICC, IHC (f), IP, RIA, WB
Citations:
37
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
B92, monoclonal
form
buffered aqueous solution
species reactivity
mouse, human
packaging
antibody small pack of 25 μL
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable, immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, microarray: suitable, radioimmunoassay: suitable using cells, western blot: 2-4 μg/mL using whole extract of cultured HeLa cells
isotype
IgG1
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TAP1(6890)
関連するカテゴリー
General description
Monoclonal Anti-PSF (mouse IgG1 isotype) is derived from the B92 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a C57BL/6J mouse immunized with a lysate of mouse bone marrow-derived stromal cell line. Polypyrimidine tract-binding protein-associated splicing factor (PSF) is an ubiquitous nuclear matrix protein. It is characterized with two consensus RNA-binding domains and an unusual amino terminus rich in proline and glutamine residues.
Immunogen
マウス骨髄由来間質細胞株のライセ-ト。
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunoprecipitation (1 paper)
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunoprecipitation (1 paper)
Monoclonal Anti-PSF antibody produced in mouse has been used in:
- immunofluorescence
- immunohistochemistry
- immunoprecipitation
Biochem/physiol Actions
Polypyrimidine tract-binding protein-associated splicing factor (PSF) plays a pivotal role in mammalian spliceosome. PSF is also necessary for pre-mRNA splicing and transcription. The restricted expression of PSF suggests that it is involved in the control of neuronal-specific splicing events occurring at particular stages of neuronal differentiation and maturation. Monoclonal antibodies reacting specifically with PSF are useful tools for the molecular identification and characterization of the functional activity of PSF.
Physical form
0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。
Preparation Note
バイオリアクタ-で増殖させたハイブリド-マ細胞の培養上清から精製しています。
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
P2860-25UL: + P2860-100UL: + P2860-VAR: + P2860-BULK: + P2860-.2ML:
jan
Christopher A Yarosh et al.
Nucleic acids research, 43(18), 9006-9016 (2015-08-12)
PSF (a.k.a. SFPQ) is a ubiquitously expressed, essential nuclear protein with important roles in DNA damage repair and RNA biogenesis. In stimulated T cells, PSF binds to and suppresses the inclusion of CD45 exon 4 in the final mRNA; however
Heike J Wobst et al.
Frontiers in molecular neuroscience, 10, 46-46 (2017-03-14)
Mutations in the gene
Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing
Morozumi Y, et al.
Nucleic Acids Research, 40(7), 3031-3041 (2011)
Wen Shen et al.
Nucleic acids research, 46(5), 2204-2217 (2018-02-02)
We reported previously that a 2' fluoro-modified (2' F) phosphorothioate (PS) antisense oligonucleotides (ASOs) with 5-10-5 gapmer configuration interacted with proteins from Drosophila behavior/human splicing (DBHS) family with higher affinity than PS-ASOs modified with 2'-O-(2-methoxyethyl) (2' MOE) or 2',4'-constrained 2'-O-ethyl
Cytoplasmic Relocalization of TAR DNA-Binding Protein 43 Is Not Sufficient to Reproduce Cellular Pathologies Associated with ALS In vitro
Wobst HJ, et al.
Frontiers in Molecular Neuroscience, 10(7) (2017)
関連コンテンツ
Instructions
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