P2860
モノクロナール抗PSF マウス宿主抗体
clone B92, purified from hybridoma cell culture
別名:
Mouse Anti-Polypyrimidine tract-binding protein-associated splicing factor
製品名
モノクロナール抗PSF マウス宿主抗体, clone B92, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
B92, monoclonal
form
buffered aqueous solution
species reactivity
mouse, human
packaging
antibody small pack of 25 μL
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
radioimmunoassay: suitable using cells
western blot: 2-4 μg/mL using whole extract of cultured HeLa cells
isotype
IgG1
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TAP1(6890)
関連するカテゴリー
Immunogen
マウス骨髄由来間質細胞株のライセ-ト。
Physical form
0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。
Preparation Note
バイオリアクタ-で増殖させたハイブリド-マ細胞の培養上清から精製しています。
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunoprecipitation (1 paper)
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunoprecipitation (1 paper)
Monoclonal Anti-PSF antibody produced in mouse has been used in:
- immunofluorescence
- immunohistochemistry
- immunoprecipitation
Biochem/physiol Actions
Polypyrimidine tract-binding protein-associated splicing factor (PSF) plays a pivotal role in mammalian spliceosome. PSF is also necessary for pre-mRNA splicing and transcription. The restricted expression of PSF suggests that it is involved in the control of neuronal-specific splicing events occurring at particular stages of neuronal differentiation and maturation. Monoclonal antibodies reacting specifically with PSF are useful tools for the molecular identification and characterization of the functional activity of PSF.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-PSF (mouse IgG1 isotype) is derived from the B92 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a C57BL/6J mouse immunized with a lysate of mouse bone marrow-derived stromal cell line. Polypyrimidine tract-binding protein-associated splicing factor (PSF) is an ubiquitous nuclear matrix protein. It is characterized with two consensus RNA-binding domains and an unusual amino terminus rich in proline and glutamine residues.
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保管分類
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Heike J Wobst et al.
Frontiers in molecular neuroscience, 10, 46-46 (2017-03-14)
Mutations in the gene
Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing
Morozumi Y, et al.
Nucleic Acids Research, 40(7), 3031-3041 (2011)
Christopher A Yarosh et al.
Nucleic acids research, 43(18), 9006-9016 (2015-08-12)
PSF (a.k.a. SFPQ) is a ubiquitously expressed, essential nuclear protein with important roles in DNA damage repair and RNA biogenesis. In stimulated T cells, PSF binds to and suppresses the inclusion of CD45 exon 4 in the final mRNA; however
Paraspeckles are subpopulation-specific nuclear bodies that are not essential in mice
Nakagawa S, et al.
The Journal of cell biology, 193(1), 31-39 (2011)
Differential nuclear localization and nuclear matrix association of the splicing factors PSF and PTB
Meissner M, et al.
Journal of Cellular Biochemistry, 76(4), 559-566 (2000)
関連コンテンツ
Instructions
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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