抗PAK1/2/3, リン酸化型(pThr⁴²³)抗体 ウサギ宿主抗体

p21-activated kinases are a family of highly conserved serine/threonine protein kinases that are activated by small GTPases of Cdc42 and Rac families. There are 2 groups of PAKs in mammals, group A and B. The group A members are three isoforms, PAK1, PAK2, and PAK3 (also known as α-PAK, γ-PAK, and β-PAK, respectively). PAKs are actively involved in regulation of MAPK pathways by phosphorylation of Raf-1 and MEK-1. They also regulate cytoskeletal reorganization by interaction with GTPases. Autophosphorylation of threonine 423 (threonine 402 for PAK2 and threonine 421 for PAK3), catalyzed by Cdc42, is required for activation of PAK.
The antibody detects human PAK1, PAK2 and PAK3, phosphorylated at Thr⁴²³. Other species with 100% PAK homology are expected to react.

PAKのスレオニン423を含む領域に由来する合成リン酸化ペプチド。

A recommended working concentration of 0.1-1.0 μg/mL is determined by immunoblotting using Histagged wild-type and S139A/T421A double mutant PAK3.

Dulbecco PBS(Mg²⁺/Ca²⁺不含)溶液(pH 7.3, 50%グリセロ-ル, 1.0 mg/mL BSA(IgGおよびプロテア-ゼフリ-), 0.05%アジ化ナトリウム含有)。

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