P7965
モノクロナール抗P-糖タンパク質(MDR) マウス宿主抗体
clone F4, ascites fluid
別名:
Anti-ABC20, Anti-CD243, Anti-CLCS, Anti-GP170, Anti-MDR1, Anti-P-GP, Anti-PGY1, Anti-p-170
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この商品について
Conjugate:
unconjugated
Clone:
F4, monoclonal
Application:
immunocytochemistry
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
immunohistochemistry (frozen sections)
immunoprecipitation (IP)
indirect ELISA
radioimmunoassay
western blot
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
immunohistochemistry (frozen sections)
immunoprecipitation (IP)
indirect ELISA
radioimmunoassay
western blot
Species reactivity:
hamster, human
Citations:
53
Technique(s):
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human kidney sections
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
radioimmunoassay: suitable using cell-surface RIA
western blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human kidney sections
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
radioimmunoassay: suitable using cell-surface RIA
western blot: suitable
Uniprot accession no.:
製品名
モノクロナール抗P-糖タンパク質(MDR) マウス宿主抗体, clone F4, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
F4, monoclonal
mol wt
antigen 170-180 kDa
contains
15 mM sodium azide
species reactivity
hamster, human
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human kidney sections
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
radioimmunoassay: suitable using cell-surface RIA
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ABCB1(5243)
関連するカテゴリー
Biochem/physiol Actions
本抗体は、P-糖タンパク質 (Pgp)の3番目の細胞外ル-プのN末端側半分にあるエピト-プを認識します。このエピト-プは、ホルマリン固定および過ヨウ素酸酸化に対して安定です。本抗体は、ヒト MDR1 P-糖タンパク質を特異的に検出しますが、ヒト MDR3、<REFERENCE ID="16647" TYPE="literature″/>マウスmdr1a、 mdr1b、mdr3 P-糖タンパク質<REFERENCE ID="16648" TYPE="literature″/>は認識しません。
Overexpression of ABCB1 (ATP binding cassette subfamily B member 1) gene is a major cause for multi-drug resistance, which makes chemotherapy challenging for the treatment of osteosarcoma. P-glycoprotein (P-gp) mediates the energy-dependent efflux of xenobiotic to the outside of plasma membrane from the inside of the cell , thereby affecting the process of absorption, distribution, and excretion of drugs. Cyclosporine, a calcineurin inhibitor serves as a substrate for P-gp.
Immunogen
ヒトおよびハムスタ-の薬剤耐性全細胞および未精製の細胞膜の混合物です。
Application
Monoclonal Anti-P-Glycoprotein (MDR) antibody produced in mouse has been used in western blot analysis and Immunohistochemistry.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
The ABCB1 (ATP binding cassette subfamily B member 1) gene is mapped to human chromosome 7q21.12. It encodes for P-glycoprotein, which is a membrane bound drug transporter protein, belonging to the ATP-binding cassette (ABC) transporters family. The gene is considered to be highly polymorphic.
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保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ABCB1 polymorphism predicts escitalopram dose needed for remission in major depression.
Singh A B, et al.
Translational Psychiatry, 2(11), e198-e198 (2012)
Yun-Kai Zhang et al.
Oncotarget, 6(27), 24277-24290 (2015-08-25)
Overexpression of ATP-Binding Cassette transporters leads to multidrug resistance in cancer cells and results in the failure of chemotherapy. In this in-vitro study, we investigated whether or not (20S, 24R/S)-epoxy-12β, 25-dihydroxy-dommarane-3β-amine (ORA and OSA), a pair of semi-synthetic ocotillol analogue
Targeting ABCB1 (MDR1) in multi-drug resistant osteosarcoma cells using the CRISPR-Cas9 system to reverse drug resistance.
Liu T, et al.
Oncotarget, 7(50), 83502-83502 (2016)
T M Chu et al.
Hybridoma, 12(4), 417-429 (1993-08-01)
Using viable adriamycin resistant human ovarian carcinoma cells 2780AD and colchicine resistant human oral epidermoid carcinoma cells KB-24 as the immunogen in primary and subsequent i.p. immunizations, followed by i.v. boostings with crude plasma membranes of 2780AD, KB-24, Chinese hamster
T M Chu et al.
Biochemical and biophysical research communications, 203(1), 506-512 (1994-08-30)
Multidrug resistance (MDR) is a unique phenomenon in cancer patients and is commonly associated with an overexpression of the human MDR gene mdr1, which encodes an energy-dependent Mr 180 kDa membrane bound protein, known as P-glycoprotein. P-glycoprotein serves as a
資料
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
関連コンテンツ
Datasheet
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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